PCR amplification of crude microbial DNA extracted from soil

C. Yeates*, M. R. Gillings, A. D. Davison, N. Altavilla, D. A. Veal

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

82 Citations (Scopus)

Abstract

A rapid, inexpensive, large-scale DNA extraction method involving minimal purification has been developed that is applicable to various soil types. DNA was extracted from 100 g of soil using direct lysis with glass beads and sodium dodecyl sulphate (SDS) followed by polyethylene glycol precipitation, potassium acetate precipitation, phenol extraction and isopropanol precipitation. The crude extract could be used in PCR directed at high-copy number (bacterial small subunit rRNA) and single-copy (fungal β-tubulin) genes.

Original languageEnglish
Pages (from-to)303-307
Number of pages5
JournalLetters in Applied Microbiology
Volume25
Issue number4
Publication statusPublished - 1997

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