PCR amplification of crude microbial DNA extracted from soil

C. Yeates; M. R. Gillings; A. D. Davison; N. Altavilla; D. A. Veal

PCR amplification of crude microbial DNA extracted from soil

C. Yeates^*, M. R. Gillings, A. D. Davison, N. Altavilla, D. A. Veal

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

97 Citations (Scopus)

Abstract

A rapid, inexpensive, large-scale DNA extraction method involving minimal purification has been developed that is applicable to various soil types. DNA was extracted from 100 g of soil using direct lysis with glass beads and sodium dodecyl sulphate (SDS) followed by polyethylene glycol precipitation, potassium acetate precipitation, phenol extraction and isopropanol precipitation. The crude extract could be used in PCR directed at high-copy number (bacterial small subunit rRNA) and single-copy (fungal β-tubulin) genes.

Original language	English
Pages (from-to)	303-307
Number of pages	5
Journal	Letters in Applied Microbiology
Volume	25
Issue number	4
Publication status	Published - 1997

Cite this

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title = "PCR amplification of crude microbial DNA extracted from soil",

abstract = "A rapid, inexpensive, large-scale DNA extraction method involving minimal purification has been developed that is applicable to various soil types. DNA was extracted from 100 g of soil using direct lysis with glass beads and sodium dodecyl sulphate (SDS) followed by polyethylene glycol precipitation, potassium acetate precipitation, phenol extraction and isopropanol precipitation. The crude extract could be used in PCR directed at high-copy number (bacterial small subunit rRNA) and single-copy (fungal β-tubulin) genes.",

author = "C. Yeates and Gillings, {M. R.} and Davison, {A. D.} and N. Altavilla and Veal, {D. A.}",

year = "1997",

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T1 - PCR amplification of crude microbial DNA extracted from soil

AU - Yeates, C.

AU - Gillings, M. R.

AU - Davison, A. D.

AU - Altavilla, N.

AU - Veal, D. A.

PY - 1997

Y1 - 1997

N2 - A rapid, inexpensive, large-scale DNA extraction method involving minimal purification has been developed that is applicable to various soil types. DNA was extracted from 100 g of soil using direct lysis with glass beads and sodium dodecyl sulphate (SDS) followed by polyethylene glycol precipitation, potassium acetate precipitation, phenol extraction and isopropanol precipitation. The crude extract could be used in PCR directed at high-copy number (bacterial small subunit rRNA) and single-copy (fungal β-tubulin) genes.

AB - A rapid, inexpensive, large-scale DNA extraction method involving minimal purification has been developed that is applicable to various soil types. DNA was extracted from 100 g of soil using direct lysis with glass beads and sodium dodecyl sulphate (SDS) followed by polyethylene glycol precipitation, potassium acetate precipitation, phenol extraction and isopropanol precipitation. The crude extract could be used in PCR directed at high-copy number (bacterial small subunit rRNA) and single-copy (fungal β-tubulin) genes.

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M3 - Article

C2 - 9351282

AN - SCOPUS:0030821740

SN - 0266-8254

VL - 25

SP - 303

EP - 307

JO - Letters in Applied Microbiology

JF - Letters in Applied Microbiology

IS - 4

ER -

PCR amplification of crude microbial DNA extracted from soil

Abstract

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