Peptide nucleic acid probes with charged photocleavable mass markers: towards PNA-based MALDI-TOF MS genetic analysis

Rachel J. Ball; Philip S. Green; Nittaya Gale; G. John Langley; Tom Brown

doi:10.4161/adna.1.1.12199

Peptide nucleic acid probes with charged photocleavable mass markers: towards PNA-based MALDI-TOF MS genetic analysis

Rachel J. Ball, Philip S. Green, Nittaya Gale, G. John Langley, Tom Brown^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

3 Citations (Scopus)

44 Downloads (Pure)

Abstract

Halogen-labelled peptide organic acid (hPOA) monomers have been synthesised and incorporated into sequence-specific peptide nucleic acid (PNA) probes. Three different types of probe have been prepared; the unmodified PNA probe, the PNA probe with a mass marker, and the PNA probe with photocleavable mass marker. All three types of probe have been used in model studies to develop a mass spectrometry-based hybridisation assay for detection of point mutations in DNA.

Original language	English
Pages (from-to)	27-35
Number of pages	9
Journal	Artificial DNA: PNA and XNA
Volume	1
Issue number	1
DOIs	https://doi.org/10.4161/adna.1.1.12199
Publication status	Published - 2010
Externally published	Yes

Bibliographical note

Copyright the Publisher 2010. Version archived for private and non-commercial use with the permission of the author/s and according to publisher conditions. For further rights please contact the publisher.

Keywords

peptide nucleic acid
photocleavable mass marker tag
genetic analysis
halogen-labelled peptide organic acid
SNP

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10.4161/adna.1.1.12199Licence: CC BY-NC

Publisher version (open access)Final published version, 1.48 MBLicence: CC BY-NC

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AU - Langley, G. John

AU - Brown, Tom

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Peptide nucleic acid probes with charged photocleavable mass markers: towards PNA-based MALDI-TOF MS genetic analysis

Abstract

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