Peptide nucleic acid probes with charged photocleavable mass markers

towards PNA-based MALDI-TOF MS genetic analysis

Rachel J. Ball, Philip S. Green, Nittaya Gale, G. John Langley, Tom Brown*

*Corresponding author for this work

Research output: Contribution to journalArticle

3 Citations (Scopus)
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Abstract

Halogen-labelled peptide organic acid (hPOA) monomers have been synthesised and incorporated into sequence-specific peptide nucleic acid (PNA) probes. Three different types of probe have been prepared; the unmodified PNA probe, the PNA probe with a mass marker, and the PNA probe with photocleavable mass marker. All three types of probe have been used in model studies to develop a mass spectrometry-based hybridisation assay for detection of point mutations in DNA.

Original languageEnglish
Pages (from-to)27-35
Number of pages9
JournalArtificial DNA: PNA and XNA
Volume1
Issue number1
DOIs
Publication statusPublished - 2010
Externally publishedYes

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Bibliographical note

Copyright the Publisher 2010. Version archived for private and non-commercial use with the permission of the author/s and according to publisher conditions. For further rights please contact the publisher.

Keywords

  • peptide nucleic acid
  • photocleavable mass marker tag
  • genetic analysis
  • halogen-labelled peptide organic acid
  • SNP

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