Halogen-labelled peptide organic acid (hPOA) monomers have been synthesised and incorporated into sequence-specific peptide nucleic acid (PNA) probes. Three different types of probe have been prepared; the unmodified PNA probe, the PNA probe with a mass marker, and the PNA probe with photocleavable mass marker. All three types of probe have been used in model studies to develop a mass spectrometry-based hybridisation assay for detection of point mutations in DNA.
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- peptide nucleic acid
- photocleavable mass marker tag
- genetic analysis
- halogen-labelled peptide organic acid