Phagocytosis of the protozoan parasite, Marteilia sydneyi, by Sydney rock oyster (Saccostrea glomerata) hemocytes

Rhiannon P. Kuchel*, Saleem Aladaileh, Debra Birch, Nicole Vella, David A. Raftos

*Corresponding author for this work

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

QX disease is a fatal disease in Sydney rock oysters caused by the protozoan parasite Marteilia sydneyi. The current study investigates the phagocytosis of M. sydneyi by Sydney rock oyster hemocytes. It also compares the in vitro phagocytic activities of hemocytes from oysters bred for QX disease resistance (QXR) with those of wild-type oysters. After ingestion of M. sydneyi, hemocyte granules fused with phagosome membranes and the pH of phagosomes decreased. Significantly (p=<0.05) more phagosomes in QXR hemocytes showed obvious changes in pH within 40. min of phagocytosis, when compared with wild-type hemocytes. Phenoloxidase deposition was also evident in phagosomes after in vitro phagocytosis. Most importantly, ingested and melanised M. sydneyi were detected in vivo among hemocytes from infected oysters. Overall, the data suggest that Sydney rock oyster hemocytes can recognise and phagocytose M. sydneyi, and that resistance against QX disease may be associated with enhanced phagolysosomal activity in QXR oysters.

Original languageEnglish
Pages (from-to)97-104
Number of pages8
JournalJournal of Invertebrate Pathology
Volume104
Issue number2
DOIs
Publication statusPublished - Jun 2010

Keywords

  • Saccostrea glomerata
  • Marteilia sydneyi
  • phagocytosis
  • phenoloxidase
  • QX disease
  • hemocytes
  • Bivalve mollusc

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