TY - JOUR
T1 - Pharmacokinetics, tissue distribution, and plasma protein binding study of chicoric acid by HPLC-MS/MS
AU - Wang, Yutang
AU - Xie, Guo
AU - Liu, Qian
AU - Duan, Xiang
AU - Liu, Zhigang
AU - Liu, Xuebo
PY - 2016/9/15
Y1 - 2016/9/15
N2 - Chicoric acid is a major active constituent of Echinacea purpurea and has a variety of biological functions. In this study, a liquid chromatography coupled with tandem mass spectrometry (HPLC–MS/MS) approach was developed and validated for the determination of chicoric acid in rat plasma and various tissues using ferulic acid as an internal standard (IS). This method was successfully applied to pharmacokinetics, tissue distribution, and plasma protein binding (PPB) study of chicoric acid in Sprague-Dawley (SD) rats dosed with 50 mg/kg by gastric gavage. The pharmacokinetic parameters were determined and showed a half-life (t
1/2) of 4.53 ± 1.44 h, an apparent volume of mean residual time (MRT) of 18.58 ± 4.43 h, and an area under the curve (AUC) of 26.14 mg h L
−1. The tissue distribution of chicoric acid in rats after gavage administration showed a decreasing tendency in different tissues (liver > lung > kidney > heart > spleen > brain). The PPB rates in rat plasma, human plasma, and bovine serum albumin were 98.3, 96.9, and 96.6%, respectively. These results provide insight for the further pharmacological investigation of chicoric acid.
AB - Chicoric acid is a major active constituent of Echinacea purpurea and has a variety of biological functions. In this study, a liquid chromatography coupled with tandem mass spectrometry (HPLC–MS/MS) approach was developed and validated for the determination of chicoric acid in rat plasma and various tissues using ferulic acid as an internal standard (IS). This method was successfully applied to pharmacokinetics, tissue distribution, and plasma protein binding (PPB) study of chicoric acid in Sprague-Dawley (SD) rats dosed with 50 mg/kg by gastric gavage. The pharmacokinetic parameters were determined and showed a half-life (t
1/2) of 4.53 ± 1.44 h, an apparent volume of mean residual time (MRT) of 18.58 ± 4.43 h, and an area under the curve (AUC) of 26.14 mg h L
−1. The tissue distribution of chicoric acid in rats after gavage administration showed a decreasing tendency in different tissues (liver > lung > kidney > heart > spleen > brain). The PPB rates in rat plasma, human plasma, and bovine serum albumin were 98.3, 96.9, and 96.6%, respectively. These results provide insight for the further pharmacological investigation of chicoric acid.
KW - chicoric acid
KW - HPLC–MS/MS
KW - pharmacokinetics
KW - tissue distribution
KW - plasma protein binding
UR - http://www.scopus.com/inward/record.url?scp=84994752259&partnerID=8YFLogxK
U2 - 10.1016/j.jchromb.2016.07.045
DO - 10.1016/j.jchromb.2016.07.045
M3 - Article
C2 - 27479684
SN - 1570-0232
VL - 1031
SP - 139
EP - 145
JO - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
JF - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
ER -