Phenoloxidase and QX disease resistance in Sydney rock oysters (Saccostrea glomerata)

Kathryn Newton; Rodney Peters; David Raftos

doi:10.1016/j.dci.2003.10.004

Phenoloxidase and QX disease resistance in Sydney rock oysters (Saccostrea glomerata)

Kathryn Newton, Rodney Peters, David Raftos^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

67 Citations (Scopus)

Abstract

QX is a fatal disease in Sydney rock oysters (Saccostrea glomerata) that results from infection by the protistan parasite, Marteilia sydneyi. Since 1997, the New South Wales Fisheries Service has bred S. glomerata for resistance to QX disease. The current study shows that the QX resistance breeding program has selected oysters with enhanced phenoloxidase (PO) activities. The third generation of QX-selected oysters was compared to S. glomerata that had never been selected for disease resistance. PO enzyme assays showed that oysters bred for resistance had significantly higher PO activities than the non-selected population. There was no difference between populations in the activities of a variety of other enzymes. Native polyacrylamide gel electrophoresis identified a novel form of PO in QX-selected oysters that contributes to their enhanced PO activities. This novel form of PO may represent a specific QX disease resistance factor.

Original language	English
Pages (from-to)	565-569
Number of pages	5
Journal	Developmental and Comparative Immunology
Volume	28
Issue number	6
DOIs	https://doi.org/10.1016/j.dci.2003.10.004
Publication status	Published - 17 May 2004

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title = "Phenoloxidase and QX disease resistance in Sydney rock oysters (Saccostrea glomerata)",

abstract = "QX is a fatal disease in Sydney rock oysters (Saccostrea glomerata) that results from infection by the protistan parasite, Marteilia sydneyi. Since 1997, the New South Wales Fisheries Service has bred S. glomerata for resistance to QX disease. The current study shows that the QX resistance breeding program has selected oysters with enhanced phenoloxidase (PO) activities. The third generation of QX-selected oysters was compared to S. glomerata that had never been selected for disease resistance. PO enzyme assays showed that oysters bred for resistance had significantly higher PO activities than the non-selected population. There was no difference between populations in the activities of a variety of other enzymes. Native polyacrylamide gel electrophoresis identified a novel form of PO in QX-selected oysters that contributes to their enhanced PO activities. This novel form of PO may represent a specific QX disease resistance factor.",

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T1 - Phenoloxidase and QX disease resistance in Sydney rock oysters (Saccostrea glomerata)

AU - Newton, Kathryn

AU - Peters, Rodney

AU - Raftos, David

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N2 - QX is a fatal disease in Sydney rock oysters (Saccostrea glomerata) that results from infection by the protistan parasite, Marteilia sydneyi. Since 1997, the New South Wales Fisheries Service has bred S. glomerata for resistance to QX disease. The current study shows that the QX resistance breeding program has selected oysters with enhanced phenoloxidase (PO) activities. The third generation of QX-selected oysters was compared to S. glomerata that had never been selected for disease resistance. PO enzyme assays showed that oysters bred for resistance had significantly higher PO activities than the non-selected population. There was no difference between populations in the activities of a variety of other enzymes. Native polyacrylamide gel electrophoresis identified a novel form of PO in QX-selected oysters that contributes to their enhanced PO activities. This novel form of PO may represent a specific QX disease resistance factor.

AB - QX is a fatal disease in Sydney rock oysters (Saccostrea glomerata) that results from infection by the protistan parasite, Marteilia sydneyi. Since 1997, the New South Wales Fisheries Service has bred S. glomerata for resistance to QX disease. The current study shows that the QX resistance breeding program has selected oysters with enhanced phenoloxidase (PO) activities. The third generation of QX-selected oysters was compared to S. glomerata that had never been selected for disease resistance. PO enzyme assays showed that oysters bred for resistance had significantly higher PO activities than the non-selected population. There was no difference between populations in the activities of a variety of other enzymes. Native polyacrylamide gel electrophoresis identified a novel form of PO in QX-selected oysters that contributes to their enhanced PO activities. This novel form of PO may represent a specific QX disease resistance factor.

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JO - Developmental and Comparative Immunology

JF - Developmental and Comparative Immunology

IS - 6

ER -

Phenoloxidase and QX disease resistance in Sydney rock oysters (Saccostrea glomerata)

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