Phosphorylated Peptide Functionalization of Lanthanide Upconversion Nanoparticles for Tuning Nanomaterial-Cell Interactions

Chi Yao, Caiyi Wei, Zhi Huang, Yiqing Lu, Ahmed Mohamed El-Toni, Dianwen Ju, Xiangmin Zhang, Wenning Wang*, Fan Zhang

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

22 Citations (Scopus)

Abstract

Peptide modification of nanoparticles with high efficiency is critical in determining the properties and bioapplications of nanoparticles, but the methodology remains a challenging task. Here, by using the phosphorylated linear and cyclic peptide with the arginine-glycine-aspartic acid (RGD) targeting motifs as typical examples, the peptides binding efficiency for the inorganic metal compound nanoparticles was increased significantly after the phosphorylation treatment, and the modification allowed for improving the selectivity and signal-to-noise ratio for cancer targeting and reduced the toxicity derived from nonspecific interactions of nanoparticles with cells owing to the higher amount of phosphopeptide binding. In addition, molecular dynamics (MD) simulations of various peptides on inorganic metal compound surfaces revealed that the peptide adsorption on the surface is mainly driven by electrostatic interactions between phosphate oxygen and the polarized interfacial water layer, consistent with the experimental observation of the strong binding propensity of phosphorylated peptides. Significantly, with the RGD phosphopeptide surface modification, these nanoparticles provide a versatile tool for tuning material-cell interactions to achieve the desired level of autophagy and may prove useful for various diagnostic and therapeutic applications.

Original languageEnglish
Pages (from-to)6935-6943
Number of pages9
JournalACS Applied Materials and Interfaces
Volume8
Issue number11
DOIs
Publication statusPublished - 23 Mar 2016

Keywords

  • lanthanide upconversion nanoparticles
  • peptide
  • phosphorylation
  • cancer target
  • autophagy

Fingerprint

Dive into the research topics of 'Phosphorylated Peptide Functionalization of Lanthanide Upconversion Nanoparticles for Tuning Nanomaterial-Cell Interactions'. Together they form a unique fingerprint.

Cite this