Photosystem II activity and pigment-protein complexes in flashed bean leaves

R. G. Hiller; Dori Pilger; Sandra Genge

doi:10.1016/0304-4211(73)90036-9

Photosystem II activity and pigment-protein complexes in flashed bean leaves

R. G. Hiller^*, Dori Pilger, Sandra Genge

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

20 Citations (Scopus)

Abstract

Bean leaves exposed to cycles of 2 min light-118 min dark, (1) can photoreduce cytochrome f; (2) have cytochrome b-559_HP; (3) have a DCMU (3-[3′,4′-dichlorophenyl]-1,1-dimethylurea)-sensitive Hill reaction. Detergent extracts of the plastids show only pigment-protein complex I. Shortening the dark period leads to formation of chl b (chlorophyll b) and the appearance of pigment-protein complex II with no increase in the rate of Hill reaction. As the proteins of detergent solubilised etioplasts and of plastids from leaves flashed for 60 h are very similar, it is concluded that pigment is added on to the pre-existing proteins of the etioplast. The presence of pigment-protein complex II reflects the requirement for chl b to stabilise it during extraction rather than the activity of PS II (Photosystem II).

Original language	English
Pages (from-to)	81-88
Number of pages	8
Journal	Plant Science Letters
Volume	1
Issue number	3
DOIs	https://doi.org/10.1016/0304-4211(73)90036-9
Publication status	Published - 1973

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title = "Photosystem II activity and pigment-protein complexes in flashed bean leaves",

abstract = "Bean leaves exposed to cycles of 2 min light-118 min dark, (1) can photoreduce cytochrome f; (2) have cytochrome b-559HP; (3) have a DCMU (3-[3′,4′-dichlorophenyl]-1,1-dimethylurea)-sensitive Hill reaction. Detergent extracts of the plastids show only pigment-protein complex I. Shortening the dark period leads to formation of chl b (chlorophyll b) and the appearance of pigment-protein complex II with no increase in the rate of Hill reaction. As the proteins of detergent solubilised etioplasts and of plastids from leaves flashed for 60 h are very similar, it is concluded that pigment is added on to the pre-existing proteins of the etioplast. The presence of pigment-protein complex II reflects the requirement for chl b to stabilise it during extraction rather than the activity of PS II (Photosystem II).",

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T1 - Photosystem II activity and pigment-protein complexes in flashed bean leaves

AU - Hiller, R. G.

AU - Pilger, Dori

AU - Genge, Sandra

PY - 1973

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N2 - Bean leaves exposed to cycles of 2 min light-118 min dark, (1) can photoreduce cytochrome f; (2) have cytochrome b-559HP; (3) have a DCMU (3-[3′,4′-dichlorophenyl]-1,1-dimethylurea)-sensitive Hill reaction. Detergent extracts of the plastids show only pigment-protein complex I. Shortening the dark period leads to formation of chl b (chlorophyll b) and the appearance of pigment-protein complex II with no increase in the rate of Hill reaction. As the proteins of detergent solubilised etioplasts and of plastids from leaves flashed for 60 h are very similar, it is concluded that pigment is added on to the pre-existing proteins of the etioplast. The presence of pigment-protein complex II reflects the requirement for chl b to stabilise it during extraction rather than the activity of PS II (Photosystem II).

AB - Bean leaves exposed to cycles of 2 min light-118 min dark, (1) can photoreduce cytochrome f; (2) have cytochrome b-559HP; (3) have a DCMU (3-[3′,4′-dichlorophenyl]-1,1-dimethylurea)-sensitive Hill reaction. Detergent extracts of the plastids show only pigment-protein complex I. Shortening the dark period leads to formation of chl b (chlorophyll b) and the appearance of pigment-protein complex II with no increase in the rate of Hill reaction. As the proteins of detergent solubilised etioplasts and of plastids from leaves flashed for 60 h are very similar, it is concluded that pigment is added on to the pre-existing proteins of the etioplast. The presence of pigment-protein complex II reflects the requirement for chl b to stabilise it during extraction rather than the activity of PS II (Photosystem II).

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DO - 10.1016/0304-4211(73)90036-9

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JO - Plant Science Letters

JF - Plant Science Letters

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Photosystem II activity and pigment-protein complexes in flashed bean leaves

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