Phototracking vaccinia virus transport reveals dynamics of cytoplasmic dispersal and a requirement for A36R and F12L for exit from the site of wrapping

Helena Lynn, Liam M. Howell, Russell J. Diefenbach, Timothy P. Newsome

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Abstract

The microtubule cytoskeleton is a primary organizer of viral infections for delivering virus particles to their sites of replication, establishing and maintaining subcellular compartments where distinct steps of viral morphogenesis take place, and ultimately dispersing viral progeny. One of the best characterized examples of virus motility is the anterograde transport of the wrapped virus form of vaccinia virus (VACV) from the trans-Golgi network (TGN) to the cell periphery by kinesin-1. Yet many aspects of this transport event are elusive due to the speed of motility and the challenges of imaging this stage at high resolution over extended time periods. We have established a novel imaging technology to track virus transport that uses photoconvertible fluorescent recombinant viruses to track subsets of virus particles from their site of origin and determine their destination. Here we image virus exit from the TGN and their rate of egress to the cell periphery. We demonstrate a role for kinesin-1 engagement in regulating virus exit from the TGN by removing A36 and F12 function, critical viral mediators of kinesin-1 recruitment to virus particles. Phototracking viral particles and components during infection is a powerful new imaging approach to elucidate mechanisms of virus replication.
Original languageEnglish
Article number390
Pages (from-to)1-18
Number of pages18
JournalViruses
Volume10
Issue number8
DOIs
Publication statusPublished - 24 Jul 2018

Bibliographical note

Copyright the Author(s) 2018. Version archived for private and non-commercial use with the permission of the author/s and according to publisher conditions. For further rights please contact the publisher.

Keywords

  • Vaccinia virus
  • Kinesin
  • Cytoskeleton
  • mircotubules
  • transport
  • photoconvertible fluorescent proteins
  • Photoconvertible fluorescent proteins
  • Microtubule
  • Transport

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