Phylogeny and lipid composition of Thermonema lapsum, a thermophilic gliding bacterium

B. K C Patel; D. S. Saul; R. A. Reeves; L. C. Williams; J. E. Cavanagh; P. D. Nichols; P. L. Bergquist

Phylogeny and lipid composition of Thermonema lapsum, a thermophilic gliding bacterium

B. K C Patel^*, D. S. Saul, R. A. Reeves, L. C. Williams, J. E. Cavanagh, P. D. Nichols, P. L. Bergquist

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

4 Citations (Scopus)

Abstract

1490 nucleotides of the 16S rRNA gene of a Gram-negative, thermophilic and gliding bacterium, Thermonema lapsum, have been sequenced. Phylogenetic analysis indicates that T. lapsum is related to cytophaga-flavobacteria-bacteriodes (CFB) and is confirmed by the identification signature nucleotides that define this group. Further phylogenetic analysis indicates that T. lapsum forms the deepest branch in the CFB group; this observation was confirmed by the identification of unique nucleotide and nucleotide pairs which separate T. lapsum from all other members of this group. The phospholipid fatty acid (PLFA) profile also confirmed that T. lapsum is related to the cytophaga-flavobacteria-bacteroides group and also to selected members of the genus Flexibacter; the PLFA profile is unique to T. lapsum.

Original language	English
Pages (from-to)	313-317
Number of pages	5
Journal	FEMS Microbiology Letters
Volume	115
Issue number	2-3
Publication status	Published - 15 Jan 1994
Externally published	Yes

Keywords

162 rRNA
Fatty acid
Lipopolysaccharide
Phospholipid
Phylogeny
Thermonema lapsum
Thermophile

Cite this

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title = "Phylogeny and lipid composition of Thermonema lapsum, a thermophilic gliding bacterium",

abstract = "1490 nucleotides of the 16S rRNA gene of a Gram-negative, thermophilic and gliding bacterium, Thermonema lapsum, have been sequenced. Phylogenetic analysis indicates that T. lapsum is related to cytophaga-flavobacteria-bacteriodes (CFB) and is confirmed by the identification signature nucleotides that define this group. Further phylogenetic analysis indicates that T. lapsum forms the deepest branch in the CFB group; this observation was confirmed by the identification of unique nucleotide and nucleotide pairs which separate T. lapsum from all other members of this group. The phospholipid fatty acid (PLFA) profile also confirmed that T. lapsum is related to the cytophaga-flavobacteria-bacteroides group and also to selected members of the genus Flexibacter; the PLFA profile is unique to T. lapsum.",

keywords = "162 rRNA, Fatty acid, Lipopolysaccharide, Phospholipid, Phylogeny, Thermonema lapsum, Thermophile",

author = "Patel, {B. K C} and Saul, {D. S.} and Reeves, {R. A.} and Williams, {L. C.} and Cavanagh, {J. E.} and Nichols, {P. D.} and Bergquist, {P. L.}",

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language = "English",

volume = "115",

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journal = "FEMS Microbiology Letters",

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T1 - Phylogeny and lipid composition of Thermonema lapsum, a thermophilic gliding bacterium

AU - Patel, B. K C

AU - Saul, D. S.

AU - Reeves, R. A.

AU - Williams, L. C.

AU - Cavanagh, J. E.

AU - Nichols, P. D.

AU - Bergquist, P. L.

PY - 1994/1/15

Y1 - 1994/1/15

N2 - 1490 nucleotides of the 16S rRNA gene of a Gram-negative, thermophilic and gliding bacterium, Thermonema lapsum, have been sequenced. Phylogenetic analysis indicates that T. lapsum is related to cytophaga-flavobacteria-bacteriodes (CFB) and is confirmed by the identification signature nucleotides that define this group. Further phylogenetic analysis indicates that T. lapsum forms the deepest branch in the CFB group; this observation was confirmed by the identification of unique nucleotide and nucleotide pairs which separate T. lapsum from all other members of this group. The phospholipid fatty acid (PLFA) profile also confirmed that T. lapsum is related to the cytophaga-flavobacteria-bacteroides group and also to selected members of the genus Flexibacter; the PLFA profile is unique to T. lapsum.

AB - 1490 nucleotides of the 16S rRNA gene of a Gram-negative, thermophilic and gliding bacterium, Thermonema lapsum, have been sequenced. Phylogenetic analysis indicates that T. lapsum is related to cytophaga-flavobacteria-bacteriodes (CFB) and is confirmed by the identification signature nucleotides that define this group. Further phylogenetic analysis indicates that T. lapsum forms the deepest branch in the CFB group; this observation was confirmed by the identification of unique nucleotide and nucleotide pairs which separate T. lapsum from all other members of this group. The phospholipid fatty acid (PLFA) profile also confirmed that T. lapsum is related to the cytophaga-flavobacteria-bacteroides group and also to selected members of the genus Flexibacter; the PLFA profile is unique to T. lapsum.

KW - 162 rRNA

KW - Fatty acid

KW - Lipopolysaccharide

KW - Phospholipid

KW - Phylogeny

KW - Thermonema lapsum

KW - Thermophile

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M3 - Article

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AN - SCOPUS:0028009467

VL - 115

SP - 313

EP - 317

JO - FEMS Microbiology Letters

JF - FEMS Microbiology Letters

SN - 0378-1097

IS - 2-3

ER -

Phylogeny and lipid composition of Thermonema lapsum, a thermophilic gliding bacterium

Abstract

Keywords

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