Phylogeny of twenty Thermus isolates constructed from 16S rRNA gene sequence data

D. J. Saul; A. G. Rodrigo; R. A. Reeves; L. C. Williams; K. M. Borges; H. W. Morgan; P. L. Bergquist

Phylogeny of twenty Thermus isolates constructed from 16S rRNA gene sequence data

D. J. Saul, A. G. Rodrigo, R. A. Reeves, L. C. Williams, K. M. Borges, H. W. Morgan, P. L. Bergquist^*

^*Corresponding author for this work

Research output: Contribution to journal › Article

51 Citations (Scopus)

Abstract

The sequences of the 16S rRNA genes of 20 Thermus isolates were determined to a high fidelity by using automated DNA sequencing and fluorescent-dye- labelled primers. The strains tested included members of the three validly named Thermus species and representatives of major taxonomic clusters defined previously for this genus. The parsimony method was used to reconstruct the phylogeny of the strains from the aligned sequences, and a bootstrap analysis revealed a number of well-supported clades. Our results are not consistent with groupings inferred from numerical taxonomy data but support the conjecture that the genus Thermus contains more species than the three currently recognized species.

Original language	English
Pages (from-to)	754-760
Number of pages	7
Journal	International Journal of Systematic Bacteriology
Volume	43
Issue number	4
Publication status	Published - 1993
Externally published	Yes

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abstract = "The sequences of the 16S rRNA genes of 20 Thermus isolates were determined to a high fidelity by using automated DNA sequencing and fluorescent-dye- labelled primers. The strains tested included members of the three validly named Thermus species and representatives of major taxonomic clusters defined previously for this genus. The parsimony method was used to reconstruct the phylogeny of the strains from the aligned sequences, and a bootstrap analysis revealed a number of well-supported clades. Our results are not consistent with groupings inferred from numerical taxonomy data but support the conjecture that the genus Thermus contains more species than the three currently recognized species.",

author = "Saul, {D. J.} and Rodrigo, {A. G.} and Reeves, {R. A.} and Williams, {L. C.} and Borges, {K. M.} and Morgan, {H. W.} and Bergquist, {P. L.}",

year = "1993",

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AU - Saul, D. J.

AU - Rodrigo, A. G.

AU - Reeves, R. A.

AU - Williams, L. C.

AU - Borges, K. M.

AU - Morgan, H. W.

AU - Bergquist, P. L.

PY - 1993

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AB - The sequences of the 16S rRNA genes of 20 Thermus isolates were determined to a high fidelity by using automated DNA sequencing and fluorescent-dye- labelled primers. The strains tested included members of the three validly named Thermus species and representatives of major taxonomic clusters defined previously for this genus. The parsimony method was used to reconstruct the phylogeny of the strains from the aligned sequences, and a bootstrap analysis revealed a number of well-supported clades. Our results are not consistent with groupings inferred from numerical taxonomy data but support the conjecture that the genus Thermus contains more species than the three currently recognized species.

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