Physical and functional interaction of the p14ARF tumor suppressor with ribosomes

Helen Rizos*, Heather A. McKenzie, Ana Luisa Ayub, Sarah Woodruff, Therese M. Becker, Lyndee L. Scurr, Joachim Stahl, Richard F. Kefford

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

24 Citations (Scopus)

Abstract

Alterations in the p14ARF tumor suppressor are frequent in many human cancers and are associated with susceptibility to melanoma, pancreatic cancer, and nervous system tumors. In addition to its p53-regulatory functions, p14ARF has been shown to influence ribosome biogenesis and to regulate the endoribonuclease B23, but there remains considerable controversy about its nucleolar role. We sought to clarify the activities of p14 ARF by studying its interaction with ribosomes. We show that p14 ARF and B23 interact within the nucleolar 60 S preribosomal particle and that this interaction does not require rRNA. In contrast to previous reports, we found that expression of p14ARF does not significantly alter ribosome biogenesis but inhibits polysome formation and protein translation in vivo. These results suggest a ribosome-dependent p14 ARF pathway that regulates cell growth and thus complements p53-dependent p14ARF functions.

Original languageEnglish
Pages (from-to)38080-38088
Number of pages9
JournalJournal of Biological Chemistry
Volume281
Issue number49
DOIs
Publication statusPublished - 8 Dec 2006
Externally publishedYes

Fingerprint Dive into the research topics of 'Physical and functional interaction of the p14<sup>ARF</sup> tumor suppressor with ribosomes'. Together they form a unique fingerprint.

Cite this