Physical isolation of endospores from environmental samples by targeted lysis of vegetative cells

Tina Wunderlin, Thomas Junier, Christophe Paul, Nicole Jeanneret, Pilar Junier

    Research output: Contribution to journalArticlepeer-review

    10 Citations (Scopus)

    Abstract

    Endospore formation is a survival strategy found among some bacteria from the phylum Firmicutes. During endospore formation, these bacteria enter a morpho-physiological resting state that enhances survival under adverse environmental conditions. Even though endospore-forming Firmicutes are one of the most frequently enriched and isolated bacterial groups in culturing studies, they are often absent from diversity studies based on molecular methods. The resistance of the spore core is considered one of the factors limiting the recovery of DNA from endospores. We developed a method that takes advantage of the higher resistance of endospores to separate them from other cells in a complex microbial community using physical, enzymatic and chemical lysis methods. The endospore-only preparation thus obtained can be used for re-culturing or to perform downstream analysis such as tailored DNA extraction optimized for endospores and subsequent DNA sequencing. This method, applied to sediment samples, has allowed the enrichment of endospores and after sequencing, has revealed a large diversity of endospore-formers in freshwater lake sediments. We expect that the application of this method to other samples will yield a similar outcome.
    Original languageEnglish
    Article numbere53411
    Pages (from-to)e53411-1-e53411-7
    Number of pages7
    JournalJournal of visualized experiments : JoVE
    Issue number107
    DOIs
    Publication statusPublished - Jan 2016

    Keywords

    • environmental sciences
    • endospores
    • separation
    • vegetative cells
    • endospore-forming bacteria
    • cell lysis
    • diversity
    • microbial ecology
    • Separation
    • Environmental sciences
    • Diversity
    • Microbial ecology
    • Cell lysis
    • Issue 107
    • Endospore-forming bacteria
    • Vegetative cells
    • Endospores

    Fingerprint

    Dive into the research topics of 'Physical isolation of endospores from environmental samples by targeted lysis of vegetative cells'. Together they form a unique fingerprint.

    Cite this