Plaque Size Tool: an automated plaque analysis tool for simplifying and standardising bacteriophage plaque morphology measurements

Ellina Trofimova, Paul R. Jaschke*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

Bacteriophage plaque size measurement is essential for phage characterisation, but manual size estimation requires a considerable amount of time and effort. In order to ease the work of phage researchers, we have developed an automated command-line application called Plaque Size Tool (PST) that can detect plaques of different morphology on the images of Petri dishes and measure plaque area and diameter. Plaque size measurements using PST showed no difference to those obtained with manual plaque size measurement in Fiji, indicating future results using PST are backwards compatible with prior measurements in the literature. PST can be applied to a range of lytic bacteriophages producing oval-shaped plaques, including bull's-eye and turbid morphology. The application can also be used for titer calculation if most of the plaques are stand-alone. As laboratory automation becomes more commonplace, standardised and flexible open-source analytical tools like PST will be important parts of biofoundry and cloud lab bacteriophage workflows.

Original languageEnglish
Pages (from-to)1-5
Number of pages5
JournalVirology
Volume561
Early online date27 May 2021
DOIs
Publication statusPublished - Sep 2021

Keywords

  • Actinobacteriophages
  • Biofoundry
  • Laboratory automation
  • Microviridae
  • Phage therapy
  • Synthetic biology

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