Plasma biomarker proteins for detection of human growth hormone administration in athletes

Sock-Hwee Tan, Albert Lee, Dana Pascovici, Natasha Care, Vita Birzniece, Ken Ho, Mark P. Molloy, Alamgir Khan

Research output: Contribution to journalArticleResearchpeer-review

Abstract

Human growth hormone (GH) is a naturally occurring hormone secreted by the pituitary gland with anabolic and growth-promoting activities. Since an increased availability of recombinant GH (rGH) for the treatment of GH-deficient patients, GH has been abused in sports and it is prohibited. "GH-isoform" and "biomarkers" tests are currently available for detection of GH abuse in sports, however both methods suffer from shortcomings. Here, we report on a proteomic approach to search for novel protein biomarkers associated with rGH administration in non-elite athletes. In this study, participants received either placebo or rGH for 8 weeks, and were followed over a 6-week washout period. We used 2-D DIGE and iTRAQ LC-MS/MS analyses to expose rGH-dependent marker proteins. Eight rGH-dependent plasma proteins namely apolipoproptein-L1, alpha-HS-glycoprotein, vitamin D-binding protein, afamin, insulin-like growth factor-binding protein-3, insulin-like growth factor-binding protein-ALS, lumican and extracellular matrix proteins 1 were identified. Apolipoprotein L1 and alpha-HS-glycoprotein were validated by Western blots to confirm their identities and expression patterns in rGH- and placebo-treated subject cohorts. Independent confirmation of these putative GH-responsive biomarkers would be of value for clinical practices and may have sports anti-doping utility.
LanguageEnglish
Article number10039
Pages1-12
Number of pages12
JournalScientific Reports
Volume7
Issue number1
DOIs
Publication statusPublished - 30 Aug 2017

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Human Growth Hormone
Athletes
Growth Hormone
Blood Proteins
Biomarkers
Sports
Glycoproteins
Placebos
Vitamin D-Binding Protein
Insulin-Like Growth Factor Binding Proteins
Insulin-Like Growth Factor Binding Protein 3
Apolipoproteins
Extracellular Matrix Proteins
Pituitary Gland
Proteomics
Protein Isoforms
Proteins
Western Blotting
Hormones

Bibliographical note

Copyright the Author(s) 2017. Version archived for private and non-commercial use with the permission of the author/s and according to publisher conditions. For further rights please contact the publisher.

Cite this

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Plasma biomarker proteins for detection of human growth hormone administration in athletes. / Tan, Sock-Hwee; Lee, Albert; Pascovici, Dana; Care, Natasha; Birzniece, Vita; Ho, Ken; Molloy, Mark P.; Khan, Alamgir.

In: Scientific Reports, Vol. 7, No. 1, 10039, 30.08.2017, p. 1-12.

Research output: Contribution to journalArticleResearchpeer-review

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AU - Ho, Ken

AU - Molloy, Mark P.

AU - Khan, Alamgir

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Y1 - 2017/8/30

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