TY - JOUR
T1 - Plasma cortisol and 11-ketotestosterone enzyme immunoassay (EIA) kit validation for three fish species
T2 - The orange clownfish Amphiprion percula, the orangefin anemonefish Amphiprion chrysopterus and the blacktip reef shark Carcharhinus melanopterus
AU - Mills, S. C.
AU - Mourier, J.
AU - Galzin, R.
PY - 2010/8
Y1 - 2010/8
N2 - Commercially available enzyme immunoassay (EIA) kits were validated for measuring steroid hormone concentrations in blood plasma from three fish species: the orange clownfish Amphiprion percula, the orangefin anemonefish Amphiprion chrysopterus and the blacktip reef shark Carcharhinus melanopterus. A minimum of 5 μl plasma was required to estimate hormone concentrations with both kits. These EIA kits are a simple method requiring minimal equipment, for measuring hormone profiles under field conditions.
AB - Commercially available enzyme immunoassay (EIA) kits were validated for measuring steroid hormone concentrations in blood plasma from three fish species: the orange clownfish Amphiprion percula, the orangefin anemonefish Amphiprion chrysopterus and the blacktip reef shark Carcharhinus melanopterus. A minimum of 5 μl plasma was required to estimate hormone concentrations with both kits. These EIA kits are a simple method requiring minimal equipment, for measuring hormone profiles under field conditions.
UR - http://www.scopus.com/inward/record.url?scp=77955263726&partnerID=8YFLogxK
U2 - 10.1111/j.1095-8649.2010.02693.x
DO - 10.1111/j.1095-8649.2010.02693.x
M3 - Article
C2 - 20701653
AN - SCOPUS:77955263726
VL - 77
SP - 769
EP - 777
JO - Journal of Fish Biology
JF - Journal of Fish Biology
SN - 0022-1112
IS - 3
ER -