Plasma cortisol and 11-ketotestosterone enzyme immunoassay (EIA) kit validation for three fish species: The orange clownfish Amphiprion percula, the orangefin anemonefish Amphiprion chrysopterus and the blacktip reef shark Carcharhinus melanopterus

S. C. Mills*, J. Mourier, R. Galzin

*Corresponding author for this work

    Research output: Contribution to journalArticlepeer-review

    22 Citations (Scopus)

    Abstract

    Commercially available enzyme immunoassay (EIA) kits were validated for measuring steroid hormone concentrations in blood plasma from three fish species: the orange clownfish Amphiprion percula, the orangefin anemonefish Amphiprion chrysopterus and the blacktip reef shark Carcharhinus melanopterus. A minimum of 5 μl plasma was required to estimate hormone concentrations with both kits. These EIA kits are a simple method requiring minimal equipment, for measuring hormone profiles under field conditions.

    Original languageEnglish
    Pages (from-to)769-777
    Number of pages9
    JournalJournal of Fish Biology
    Volume77
    Issue number3
    DOIs
    Publication statusPublished - Aug 2010

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