Plasmonic approach to enhanced fluorescence for applications in biotechnology and the life sciences

Research output: Contribution to journalArticleResearchpeer-review

Abstract

One of the most rapidly growing areas of physics and nanotechnology is concerned with plasmonic effects on the nanometer scale; these have applications in sensing and imaging technologies. Nanoplasmonic colloids such as Ag and Au have been attracting active interest, and there has been a recent explosion in the use of these metallic nanostructures to modify the spectral properties of fluorophores favorably and to enhance the fluorescence emission intensity. In this feature article, we summarize our work over a range of nanoplasmonics- assisted biological applications such as flow cytometry, immunoassays, cell imaging and bioassays where we use custom-designed plasmonic nanostructures (Ag and Au) to enhance fluorescence signatures. This fluorophore-metal effect offers unique advantages in providing improved photostability and enhanced fluorescence signals. We discuss the plasmonic enhancement of lanthanide fluorophores whose long and microsecond lifetimes offer the advantage of background-free fluorescence detection, but low photon cycling rates lead to poor brightness. We also show that plasmonic colloids are capable of enhancing the emission of fluorescent nanoparticles, including upconverting nanocrystals and lanthanide nanocomposites.

LanguageEnglish
Pages10152-10163
Number of pages12
JournalLangmuir
Volume28
Issue number27
DOIs
Publication statusPublished - 10 Jul 2012

Fingerprint

biotechnology
life sciences
Biotechnology
Fluorophores
Fluorescence
Lanthanoid Series Elements
fluorescence
Colloids
Rare earth elements
colloids
Nanostructures
Imaging techniques
bioassay
immunoassay
cytometry
Flow cytometry
Bioassay
nanotechnology
Nanotechnology
Nanocrystals

Cite this

@article{d5b37246c61e4930851e398c7a943eb8,
title = "Plasmonic approach to enhanced fluorescence for applications in biotechnology and the life sciences",
abstract = "One of the most rapidly growing areas of physics and nanotechnology is concerned with plasmonic effects on the nanometer scale; these have applications in sensing and imaging technologies. Nanoplasmonic colloids such as Ag and Au have been attracting active interest, and there has been a recent explosion in the use of these metallic nanostructures to modify the spectral properties of fluorophores favorably and to enhance the fluorescence emission intensity. In this feature article, we summarize our work over a range of nanoplasmonics- assisted biological applications such as flow cytometry, immunoassays, cell imaging and bioassays where we use custom-designed plasmonic nanostructures (Ag and Au) to enhance fluorescence signatures. This fluorophore-metal effect offers unique advantages in providing improved photostability and enhanced fluorescence signals. We discuss the plasmonic enhancement of lanthanide fluorophores whose long and microsecond lifetimes offer the advantage of background-free fluorescence detection, but low photon cycling rates lead to poor brightness. We also show that plasmonic colloids are capable of enhancing the emission of fluorescent nanoparticles, including upconverting nanocrystals and lanthanide nanocomposites.",
author = "Wei Deng and Goldys, {Ewa M.}",
year = "2012",
month = "7",
day = "10",
doi = "10.1021/la300332x",
language = "English",
volume = "28",
pages = "10152--10163",
journal = "Langmuir",
issn = "0743-7463",
publisher = "American Chemical Society",
number = "27",

}

Plasmonic approach to enhanced fluorescence for applications in biotechnology and the life sciences. / Deng, Wei; Goldys, Ewa M.

In: Langmuir, Vol. 28, No. 27, 10.07.2012, p. 10152-10163.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - Plasmonic approach to enhanced fluorescence for applications in biotechnology and the life sciences

AU - Deng, Wei

AU - Goldys, Ewa M.

PY - 2012/7/10

Y1 - 2012/7/10

N2 - One of the most rapidly growing areas of physics and nanotechnology is concerned with plasmonic effects on the nanometer scale; these have applications in sensing and imaging technologies. Nanoplasmonic colloids such as Ag and Au have been attracting active interest, and there has been a recent explosion in the use of these metallic nanostructures to modify the spectral properties of fluorophores favorably and to enhance the fluorescence emission intensity. In this feature article, we summarize our work over a range of nanoplasmonics- assisted biological applications such as flow cytometry, immunoassays, cell imaging and bioassays where we use custom-designed plasmonic nanostructures (Ag and Au) to enhance fluorescence signatures. This fluorophore-metal effect offers unique advantages in providing improved photostability and enhanced fluorescence signals. We discuss the plasmonic enhancement of lanthanide fluorophores whose long and microsecond lifetimes offer the advantage of background-free fluorescence detection, but low photon cycling rates lead to poor brightness. We also show that plasmonic colloids are capable of enhancing the emission of fluorescent nanoparticles, including upconverting nanocrystals and lanthanide nanocomposites.

AB - One of the most rapidly growing areas of physics and nanotechnology is concerned with plasmonic effects on the nanometer scale; these have applications in sensing and imaging technologies. Nanoplasmonic colloids such as Ag and Au have been attracting active interest, and there has been a recent explosion in the use of these metallic nanostructures to modify the spectral properties of fluorophores favorably and to enhance the fluorescence emission intensity. In this feature article, we summarize our work over a range of nanoplasmonics- assisted biological applications such as flow cytometry, immunoassays, cell imaging and bioassays where we use custom-designed plasmonic nanostructures (Ag and Au) to enhance fluorescence signatures. This fluorophore-metal effect offers unique advantages in providing improved photostability and enhanced fluorescence signals. We discuss the plasmonic enhancement of lanthanide fluorophores whose long and microsecond lifetimes offer the advantage of background-free fluorescence detection, but low photon cycling rates lead to poor brightness. We also show that plasmonic colloids are capable of enhancing the emission of fluorescent nanoparticles, including upconverting nanocrystals and lanthanide nanocomposites.

UR - http://www.scopus.com/inward/record.url?scp=84863746460&partnerID=8YFLogxK

U2 - 10.1021/la300332x

DO - 10.1021/la300332x

M3 - Article

VL - 28

SP - 10152

EP - 10163

JO - Langmuir

T2 - Langmuir

JF - Langmuir

SN - 0743-7463

IS - 27

ER -