PLC-β1, activated via mGluRs, mediates activity-dependent differentiation in cerebral cortex

Anthony J. Hannan; Colin Blakemore; Alla Katsnelson; Tania Vitalis; Kimberly M. Huber; Mark Bear; John Roder; Daesoo Kim; Hee Sup Shin; Peter C. Kind

doi:10.1038/85132

PLC-β1, activated via mGluRs, mediates activity-dependent differentiation in cerebral cortex

Anthony J. Hannan, Colin Blakemore, Alla Katsnelson, Tania Vitalis, Kimberly M. Huber, Mark Bear, John Roder, Daesoo Kim, Hee Sup Shin, Peter C. Kind^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

196 Citations (Scopus)

Abstract

During development of the cerebral cortex, the invasion of thalamic axons and subsequent differentiation of cortical neurons are tightly coordinated. Here we provide evidence that glutamate neurotransmission triggers a critical signaling mechanism involving the activation of phospholipase C-β1 (PLC-β1) by metabotropic glutamate receptors (mGluRs). Homozygous null mutation of either PLC-β1 or mGluR5 dramatically disrupts the cytoarchitectural differentiation of 'barrels' in the mouse somatosensory cortex, despite segregation in the pattern of thalamic innervation. Furthermore, group 1 mGluR-stimulated phosphoinositide hydrolysis is dramatically reduced in PLC-β1^-/- mice during barrel development. Our data indicate that PLC-β1 activation via mGluR5 is critical for the coordinated development of the neocortex, and that presynaptic and postsynaptic components of cortical differentiation can be genetically dissociated.

Original language	English
Pages (from-to)	282-288
Number of pages	7
Journal	Nature Neuroscience
Volume	4
Issue number	3
DOIs	https://doi.org/10.1038/85132
Publication status	Published - 2001
Externally published	Yes

Access to Document

10.1038/85132

Cite this

@article{674d22c2560a4155877efd90e6fe76b6,

title = "PLC-β1, activated via mGluRs, mediates activity-dependent differentiation in cerebral cortex",

abstract = "During development of the cerebral cortex, the invasion of thalamic axons and subsequent differentiation of cortical neurons are tightly coordinated. Here we provide evidence that glutamate neurotransmission triggers a critical signaling mechanism involving the activation of phospholipase C-β1 (PLC-β1) by metabotropic glutamate receptors (mGluRs). Homozygous null mutation of either PLC-β1 or mGluR5 dramatically disrupts the cytoarchitectural differentiation of 'barrels' in the mouse somatosensory cortex, despite segregation in the pattern of thalamic innervation. Furthermore, group 1 mGluR-stimulated phosphoinositide hydrolysis is dramatically reduced in PLC-β1-/- mice during barrel development. Our data indicate that PLC-β1 activation via mGluR5 is critical for the coordinated development of the neocortex, and that presynaptic and postsynaptic components of cortical differentiation can be genetically dissociated.",

author = "Hannan, \{Anthony J.\} and Colin Blakemore and Alla Katsnelson and Tania Vitalis and Huber, \{Kimberly M.\} and Mark Bear and John Roder and Daesoo Kim and Shin, \{Hee Sup\} and Kind, \{Peter C.\}",

year = "2001",

doi = "10.1038/85132",

language = "English",

volume = "4",

pages = "282--288",

journal = "Nature Neuroscience",

issn = "1097-6256",

publisher = "Springer, Springer Nature",

number = "3",

}

TY - JOUR

T1 - PLC-β1, activated via mGluRs, mediates activity-dependent differentiation in cerebral cortex

AU - Hannan, Anthony J.

AU - Blakemore, Colin

AU - Katsnelson, Alla

AU - Vitalis, Tania

AU - Huber, Kimberly M.

AU - Bear, Mark

AU - Roder, John

AU - Kim, Daesoo

AU - Shin, Hee Sup

AU - Kind, Peter C.

PY - 2001

Y1 - 2001

N2 - During development of the cerebral cortex, the invasion of thalamic axons and subsequent differentiation of cortical neurons are tightly coordinated. Here we provide evidence that glutamate neurotransmission triggers a critical signaling mechanism involving the activation of phospholipase C-β1 (PLC-β1) by metabotropic glutamate receptors (mGluRs). Homozygous null mutation of either PLC-β1 or mGluR5 dramatically disrupts the cytoarchitectural differentiation of 'barrels' in the mouse somatosensory cortex, despite segregation in the pattern of thalamic innervation. Furthermore, group 1 mGluR-stimulated phosphoinositide hydrolysis is dramatically reduced in PLC-β1-/- mice during barrel development. Our data indicate that PLC-β1 activation via mGluR5 is critical for the coordinated development of the neocortex, and that presynaptic and postsynaptic components of cortical differentiation can be genetically dissociated.

AB - During development of the cerebral cortex, the invasion of thalamic axons and subsequent differentiation of cortical neurons are tightly coordinated. Here we provide evidence that glutamate neurotransmission triggers a critical signaling mechanism involving the activation of phospholipase C-β1 (PLC-β1) by metabotropic glutamate receptors (mGluRs). Homozygous null mutation of either PLC-β1 or mGluR5 dramatically disrupts the cytoarchitectural differentiation of 'barrels' in the mouse somatosensory cortex, despite segregation in the pattern of thalamic innervation. Furthermore, group 1 mGluR-stimulated phosphoinositide hydrolysis is dramatically reduced in PLC-β1-/- mice during barrel development. Our data indicate that PLC-β1 activation via mGluR5 is critical for the coordinated development of the neocortex, and that presynaptic and postsynaptic components of cortical differentiation can be genetically dissociated.

UR - https://www.scopus.com/pages/publications/0034745927

U2 - 10.1038/85132

DO - 10.1038/85132

M3 - Article

C2 - 11224545

AN - SCOPUS:0034745927

SN - 1097-6256

VL - 4

SP - 282

EP - 288

JO - Nature Neuroscience

JF - Nature Neuroscience

IS - 3

ER -

PLC-β1, activated via mGluRs, mediates activity-dependent differentiation in cerebral cortex

Abstract

Access to Document

Other files and links

Fingerprint

Cite this