PLGA nanocomposites loaded with verteporfin and gold nanoparticles for enhanced photodynamic therapy of cancer cells

Wei Deng, Zofia Kautzka, Wenjie Chen, Ewa M. Goldys

Research output: Contribution to journalArticleResearchpeer-review

Abstract

In this paper, PLGA nanocomposites were developed by incorporating a photosensitizer, verteporfin and gold nanoparticles into the polymeric matrix and utilised for enhanced photodynamic therapy of cancer cells. Both enhanced fluorescence and 1O2 generation from verteporfin were observed in this new formulation under both 425 nm LED and 405 nm laser illumination. A maximum enhancement factor of 2.5 for fluorescence and 1.84 for 1O2 generation was obtained when the molar ratio of gold : VP was 5 : 1 and excited at 425 nm, compared with PLGA doped with verteporfin alone. The experiment results could be explained by the local electric field enhancement of gold nanoparticles. Furthermore, improved therapeutic efficacy in human pancreatic cancer cells, PANC-1, was also demonstrated by using this new formulation following light exposure, indicating the utility of these nanocomposites for enhanced photodynamic therapy.

LanguageEnglish
Pages112393-112402
Number of pages10
JournalRSC Advances
Volume6
Issue number113
DOIs
Publication statusPublished - 2016

Fingerprint

Photodynamic therapy
Gold
Nanocomposites
Cells
Nanoparticles
Fluorescence
Photosensitizing Agents
Photosensitizers
Light emitting diodes
Lighting
Electric fields
Lasers
verteporfin
polylactic acid-polyglycolic acid copolymer
Experiments

Cite this

@article{f2fd3bcaeb314894b6b083838d54d536,
title = "PLGA nanocomposites loaded with verteporfin and gold nanoparticles for enhanced photodynamic therapy of cancer cells",
abstract = "In this paper, PLGA nanocomposites were developed by incorporating a photosensitizer, verteporfin and gold nanoparticles into the polymeric matrix and utilised for enhanced photodynamic therapy of cancer cells. Both enhanced fluorescence and 1O2 generation from verteporfin were observed in this new formulation under both 425 nm LED and 405 nm laser illumination. A maximum enhancement factor of 2.5 for fluorescence and 1.84 for 1O2 generation was obtained when the molar ratio of gold : VP was 5 : 1 and excited at 425 nm, compared with PLGA doped with verteporfin alone. The experiment results could be explained by the local electric field enhancement of gold nanoparticles. Furthermore, improved therapeutic efficacy in human pancreatic cancer cells, PANC-1, was also demonstrated by using this new formulation following light exposure, indicating the utility of these nanocomposites for enhanced photodynamic therapy.",
author = "Wei Deng and Zofia Kautzka and Wenjie Chen and Goldys, {Ewa M.}",
year = "2016",
doi = "10.1039/c6ra21997g",
language = "English",
volume = "6",
pages = "112393--112402",
journal = "RSC Advances",
issn = "2046-2069",
publisher = "Royal Society of Chemistry",
number = "113",

}

PLGA nanocomposites loaded with verteporfin and gold nanoparticles for enhanced photodynamic therapy of cancer cells. / Deng, Wei; Kautzka, Zofia; Chen, Wenjie; Goldys, Ewa M.

In: RSC Advances, Vol. 6, No. 113, 2016, p. 112393-112402.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - PLGA nanocomposites loaded with verteporfin and gold nanoparticles for enhanced photodynamic therapy of cancer cells

AU - Deng, Wei

AU - Kautzka, Zofia

AU - Chen, Wenjie

AU - Goldys, Ewa M.

PY - 2016

Y1 - 2016

N2 - In this paper, PLGA nanocomposites were developed by incorporating a photosensitizer, verteporfin and gold nanoparticles into the polymeric matrix and utilised for enhanced photodynamic therapy of cancer cells. Both enhanced fluorescence and 1O2 generation from verteporfin were observed in this new formulation under both 425 nm LED and 405 nm laser illumination. A maximum enhancement factor of 2.5 for fluorescence and 1.84 for 1O2 generation was obtained when the molar ratio of gold : VP was 5 : 1 and excited at 425 nm, compared with PLGA doped with verteporfin alone. The experiment results could be explained by the local electric field enhancement of gold nanoparticles. Furthermore, improved therapeutic efficacy in human pancreatic cancer cells, PANC-1, was also demonstrated by using this new formulation following light exposure, indicating the utility of these nanocomposites for enhanced photodynamic therapy.

AB - In this paper, PLGA nanocomposites were developed by incorporating a photosensitizer, verteporfin and gold nanoparticles into the polymeric matrix and utilised for enhanced photodynamic therapy of cancer cells. Both enhanced fluorescence and 1O2 generation from verteporfin were observed in this new formulation under both 425 nm LED and 405 nm laser illumination. A maximum enhancement factor of 2.5 for fluorescence and 1.84 for 1O2 generation was obtained when the molar ratio of gold : VP was 5 : 1 and excited at 425 nm, compared with PLGA doped with verteporfin alone. The experiment results could be explained by the local electric field enhancement of gold nanoparticles. Furthermore, improved therapeutic efficacy in human pancreatic cancer cells, PANC-1, was also demonstrated by using this new formulation following light exposure, indicating the utility of these nanocomposites for enhanced photodynamic therapy.

UR - http://www.scopus.com/inward/record.url?scp=84999233544&partnerID=8YFLogxK

UR - http://purl.org/au-research/grants/arc/DE130100894

UR - http://purl.org/au-research/grants/arc/CE140100003

U2 - 10.1039/c6ra21997g

DO - 10.1039/c6ra21997g

M3 - Article

VL - 6

SP - 112393

EP - 112402

JO - RSC Advances

T2 - RSC Advances

JF - RSC Advances

SN - 2046-2069

IS - 113

ER -