PNA Hybeacons for analysis of human mutations related to statin-induced myopathy

Nittaya Gale, Petr Kocalka, Charlotte Mardle, Tom Brown*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

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Abstract

Aminoalkyl and alkyne-tagged PNA Hybeacons have been synthesized, labeled with fluoresce in via conventional amide bond or triazole formation (click chemistry) and used to detect single nucleotide polymorphisms (SNPs) implicated in statin-induced myopathy. The PNA hyBeacons gave much better mismatch/mutant discrimination than conventional DNA hyBeacons but smaller fluorescence changes on melting.

Original languageEnglish
Pages (from-to)79-89
Number of pages11
JournalArtificial DNA: PNA and XNA
Volume2
Issue number3
DOIs
Publication statusPublished - 2011
Externally publishedYes

Bibliographical note

Copyright the Publisher 2011. Version archived for private and non-commercial use with the permission of the author/s and according to publisher conditions. For further rights please contact the publisher.

Keywords

  • PNA
  • HyBeacon
  • fluorescence melting
  • genetic analysis
  • SNPs
  • statin-induced myopathy

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