Abstract
Aminoalkyl and alkyne-tagged PNA Hybeacons have been synthesized, labeled with fluoresce in via conventional amide bond or triazole formation (click chemistry) and used to detect single nucleotide polymorphisms (SNPs) implicated in statin-induced myopathy. The PNA hyBeacons gave much better mismatch/mutant discrimination than conventional DNA hyBeacons but smaller fluorescence changes on melting.
Original language | English |
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Pages (from-to) | 79-89 |
Number of pages | 11 |
Journal | Artificial DNA: PNA and XNA |
Volume | 2 |
Issue number | 3 |
DOIs | |
Publication status | Published - 2011 |
Externally published | Yes |
Bibliographical note
Copyright the Publisher 2011. Version archived for private and non-commercial use with the permission of the author/s and according to publisher conditions. For further rights please contact the publisher.Keywords
- PNA
- HyBeacon
- fluorescence melting
- genetic analysis
- SNPs
- statin-induced myopathy