Aminoalkyl and alkyne-tagged PNA Hybeacons have been synthesized, labeled with fluoresce in via conventional amide bond or triazole formation (click chemistry) and used to detect single nucleotide polymorphisms (SNPs) implicated in statin-induced myopathy. The PNA hyBeacons gave much better mismatch/mutant discrimination than conventional DNA hyBeacons but smaller fluorescence changes on melting.
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- fluorescence melting
- genetic analysis
- statin-induced myopathy