PNA Hybeacons for analysis of human mutations related to statin-induced myopathy

Nittaya Gale; Petr Kocalka; Charlotte Mardle; Tom Brown

doi:10.4161/adna.18179

PNA Hybeacons for analysis of human mutations related to statin-induced myopathy

Nittaya Gale, Petr Kocalka, Charlotte Mardle, Tom Brown^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

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Abstract

Aminoalkyl and alkyne-tagged PNA Hybeacons have been synthesized, labeled with fluoresce in via conventional amide bond or triazole formation (click chemistry) and used to detect single nucleotide polymorphisms (SNPs) implicated in statin-induced myopathy. The PNA hyBeacons gave much better mismatch/mutant discrimination than conventional DNA hyBeacons but smaller fluorescence changes on melting.

Original language	English
Pages (from-to)	79-89
Number of pages	11
Journal	Artificial DNA: PNA and XNA
Volume	2
Issue number	3
DOIs	https://doi.org/10.4161/adna.18179
Publication status	Published - 2011
Externally published	Yes

Bibliographical note

Copyright the Publisher 2011. Version archived for private and non-commercial use with the permission of the author/s and according to publisher conditions. For further rights please contact the publisher.

Keywords

PNA
HyBeacon
fluorescence melting
genetic analysis
SNPs
statin-induced myopathy

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10.4161/adna.18179Licence: CC BY-NC

Publisher version (open access)Final published version, 1.28 MBLicence: CC BY-NC

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AU - Mardle, Charlotte

AU - Brown, Tom

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PNA Hybeacons for analysis of human mutations related to statin-induced myopathy

Abstract

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Keywords

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