Poly I:C induces a protective antiviral immune response in the Pacific oyster (Crassostrea gigas) against subsequent challenge with Ostreid herpesvirus (OsHV-1 μvar)

Timothy J. Green*, Caroline Montagnani

*Corresponding author for this work

Research output: Contribution to journalArticle

69 Citations (Scopus)

Abstract

In-vivo studies were carried out to investigate the protective effect of a synthetic viral analogue (poly I:C) against Ostreid herpes virus (OsHV-1 μvar). Pacific oysters (Crassostrea gigas) were immune-primed by intramuscular injection of 240μg of poly I:C or sterile seawater at 1 day prior to infection with OsHV-1 μvar. Poly I:C injection induced an antiviral state in C. gigas as the percentage of viral-infected oysters at 48h post infection was significantly lower in the poly I:C treatment (11%) compared to seawater controls (100%). In an additional experiment, we demonstrated that the protective role of poly I:C is reproducible and elicits a specific antiviral response as immune-priming with heat-killed Vibrio splendidus provided no protection against subsequent viral infection. In both experiments, genes homologous to a toll-like receptor (TLR), MyD88, interferon regulatory factor (IRF) and protein kinase R (PKR) were up-regulated in oysters immune-primed with poly I:C compared to seawater controls (p<0.05). The MyD88, IRF and PKR genes were also significantly up-regulated in response to OsHV-1 μvar infection (p<0.05), which is suggestive that they are implicated in the antiviral response of C. gigas. Our results demonstrate that C. gigas can recognise double-strand RNA to initiate an innate immune response that inhibits viral infection. The observed response has striking similarities to the hallmarks of the type-1 interferon response of vertebrates.

Original languageEnglish
Pages (from-to)382-388
Number of pages7
JournalFish and Shellfish Immunology
Volume35
Issue number2
DOIs
Publication statusPublished - Aug 2013
Externally publishedYes

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