Poly(dimethylsiloxane) microchip: Microchannel with integrated open electrospray tip

Malin Svedberg; Monica Veszelei; Jan Axelsson; Mattias Vangbo; Fredrik Nikolajeff

doi:10.1039/b402490g

Poly(dimethylsiloxane) microchip: Microchannel with integrated open electrospray tip

Malin Svedberg^*, Monica Veszelei, Jan Axelsson, Mattias Vangbo, Fredrik Nikolajeff

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

56 Citations (Scopus)

Abstract

A polymer microchip with an open tip for electrospray mass spectrometry is presented. The tip consists of a groove with parallel walls where a droplet can form at the end surface. A lid covers the whole chip except at the microchannel tip, which is left open. Poly(dimethylsiloxane) (PDMS) microchips were cast using a nickel mould which in turn was replicated from a dry etched silicon wafer. Tips with microchannel widths of around 50 μm could easily be replicated. Since the tip had no cover, the assembly of microchip and cover was simplified. A total ion current variation of 5% during 300 s was achieved for a 1 μM myoglobin solution. The non-complex design of the cover makes it suitable for versatile tests of chip prototypes. The nickel mould was found to be useful for PDMS microstructure fabrication. Also, such a robust mould allows casting electrospray tips in more rigid thermoset materials.

Original language	English
Pages (from-to)	322-327
Number of pages	6
Journal	Lab on a Chip - Miniaturisation for Chemistry and Biology
Volume	4
Issue number	4
DOIs	https://doi.org/10.1039/b402490g
Publication status	Published - Aug 2004

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abstract = "A polymer microchip with an open tip for electrospray mass spectrometry is presented. The tip consists of a groove with parallel walls where a droplet can form at the end surface. A lid covers the whole chip except at the microchannel tip, which is left open. Poly(dimethylsiloxane) (PDMS) microchips were cast using a nickel mould which in turn was replicated from a dry etched silicon wafer. Tips with microchannel widths of around 50 μm could easily be replicated. Since the tip had no cover, the assembly of microchip and cover was simplified. A total ion current variation of 5% during 300 s was achieved for a 1 μM myoglobin solution. The non-complex design of the cover makes it suitable for versatile tests of chip prototypes. The nickel mould was found to be useful for PDMS microstructure fabrication. Also, such a robust mould allows casting electrospray tips in more rigid thermoset materials.",

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AU - Veszelei, Monica

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AU - Vangbo, Mattias

AU - Nikolajeff, Fredrik

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Poly(dimethylsiloxane) microchip: Microchannel with integrated open electrospray tip

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