TY - JOUR
T1 - Polysaccharides from Rhizopus nigricans mycelia induced apoptosis and G2/M arrest in BGC-823 cells
AU - Chen, Guochuang
AU - Zhang, Pengying
AU - Huang, Taotao
AU - Yu, Wenqian
AU - Lin, Jun
AU - Li, Ping
AU - Chen, Kaoshan
PY - 2013
Y1 - 2013
N2 - In this study, we reported that polysaccharides (RPS) from the mycelia of liquid-cultured Rhizopus nigricans inhibited the proliferation and clonogenic potential of human gastric cancer BGC-823 cells. Results of acridine orange/ethidium bromide and Hoechst 33258 staining showed that treated cells displayed typical morphological characteristics of apoptosis such as condensation of chromatin, nuclear pyknosis and formation of apoptotic bodies. Flow cytometry analyses and colorimetric assay indicated that RPS induced mitochondria-mediated apoptosis which was associated with collapse of mitochondrial membrane potential, activation of caspase-9 and caspase-3, generation of intracellular reactive oxygen species and elevation of intracellular calcium in BGC-823 cells. Moreover, cell-cycle analysis revealed that RPS arrested cells in the G2/M phase of the cell cycle. These results provided further insights into the potential use of RPS as an anti-cancer agent against human gastric cancer.
AB - In this study, we reported that polysaccharides (RPS) from the mycelia of liquid-cultured Rhizopus nigricans inhibited the proliferation and clonogenic potential of human gastric cancer BGC-823 cells. Results of acridine orange/ethidium bromide and Hoechst 33258 staining showed that treated cells displayed typical morphological characteristics of apoptosis such as condensation of chromatin, nuclear pyknosis and formation of apoptotic bodies. Flow cytometry analyses and colorimetric assay indicated that RPS induced mitochondria-mediated apoptosis which was associated with collapse of mitochondrial membrane potential, activation of caspase-9 and caspase-3, generation of intracellular reactive oxygen species and elevation of intracellular calcium in BGC-823 cells. Moreover, cell-cycle analysis revealed that RPS arrested cells in the G2/M phase of the cell cycle. These results provided further insights into the potential use of RPS as an anti-cancer agent against human gastric cancer.
KW - Apoptosis
KW - Cell-cycle arrest
KW - Human gastric cancer
KW - Mycelia polysaccharides
KW - Rhizopus nigricans
UR - http://www.scopus.com/inward/record.url?scp=84879496839&partnerID=8YFLogxK
U2 - 10.1016/j.carbpol.2013.05.068
DO - 10.1016/j.carbpol.2013.05.068
M3 - Article
C2 - 23911518
AN - SCOPUS:84879496839
SN - 0144-8617
VL - 97
SP - 800
EP - 808
JO - Carbohydrate Polymers
JF - Carbohydrate Polymers
IS - 2
ER -