Post-translation modification of proteins in tears

Jingjing You, Anna Fitzgerald, Paul J. Cozzi, Zhenjun Zhao, Peter Graham, Pamela J. Russell, Bradley J. Walsh, Mark Willcox, Ling Zhong, Valerie Wasinger, Yong Li*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

40 Citations (Scopus)

Abstract

This is the first 2-DE study using sequential dyes to analyse phospho-, glyco- and total tear protein profiles (Pro-Q Diamond for phosphoprotein, Pro-Q Emerald for glycoprotein and Sypro Ruby for total protein). This method minimised the gel-gel variations, allowing better comparisons among the three profiles and generated a whole map of PTM profiles of tear protein. A novel tear protein, dermcidin, was identified for the first time in this study. The identification of this antimicrobial protein suggests a new model of defence in tears. In addition, we are able to present the first experimental evidence of the presence of glycosylated lipocalin 1 and cystatin S. Nucleobindin 2 was only detected using phospho staining, suggesting it is only phosphorylated in tears. This study provides the groundwork for understanding the PTM of tear proteins and consequently these methods could be useful in the search for biomarkers in tears.

Original languageEnglish
Pages (from-to)1853-1861
Number of pages9
JournalElectrophoresis
Volume31
Issue number11
DOIs
Publication statusPublished - Jun 2010
Externally publishedYes

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