TY - JOUR
T1 - Potential analgesic, anti-inflammatory and antioxidant activities of hydroalcoholic extract of Areca catechu L. nut
AU - Bhandare, Amol M.
AU - Kshirsagar, Ajay D.
AU - Vyawahare, Neeraj S.
AU - Hadambar, Avinash A.
AU - Thorve, Vrushali S.
PY - 2010/12
Y1 - 2010/12
N2 - The hydroalcoholic extract of Areca catechu L. (ANE) nut was screened for its analgesic, anti-inflammatory and in vitro antioxidant potential. Three doses of ANE (250, 500 and 1000mg/kg orally) were tested for analgesic and anti-inflammatory activities. Evaluation of analgesic activity of ANE was performed using hot plate and formalin test in mice. ANE showed maximum increase in hot plate reaction time (56.27%, p<0.01), while reduced the duration of licking/biting behaviors in first (39.45%, p<0.05) and second (92.71%, p<0.01) phases of the formalin test indicating significant analgesic activity. ANE reduced the paw edema considerably (86.79% inhibition after 24h, p<0.01) in dose-dependent manner compared to carrageenan-induced rat. In addition, in vitro antioxidant activity of ANE was investigated by total phenolic content (TPC) and hydrogen peroxide assay. The IC50 observed in hydrogen peroxide assay was 83.14μg/ml and TPC 120.56±21.09mg QE/g. Altogether, these results suggest that the hydroalcoholic extract of Areca catechu could be considered as a potential analgesic, anti-inflammatory and antioxidant agent.
AB - The hydroalcoholic extract of Areca catechu L. (ANE) nut was screened for its analgesic, anti-inflammatory and in vitro antioxidant potential. Three doses of ANE (250, 500 and 1000mg/kg orally) were tested for analgesic and anti-inflammatory activities. Evaluation of analgesic activity of ANE was performed using hot plate and formalin test in mice. ANE showed maximum increase in hot plate reaction time (56.27%, p<0.01), while reduced the duration of licking/biting behaviors in first (39.45%, p<0.05) and second (92.71%, p<0.01) phases of the formalin test indicating significant analgesic activity. ANE reduced the paw edema considerably (86.79% inhibition after 24h, p<0.01) in dose-dependent manner compared to carrageenan-induced rat. In addition, in vitro antioxidant activity of ANE was investigated by total phenolic content (TPC) and hydrogen peroxide assay. The IC50 observed in hydrogen peroxide assay was 83.14μg/ml and TPC 120.56±21.09mg QE/g. Altogether, these results suggest that the hydroalcoholic extract of Areca catechu could be considered as a potential analgesic, anti-inflammatory and antioxidant agent.
KW - Analgesic
KW - Anti-inflammatory
KW - Antioxidant
KW - Areca catechu
KW - Arecoline
UR - http://www.scopus.com/inward/record.url?scp=78049448738&partnerID=8YFLogxK
U2 - 10.1016/j.fct.2010.09.013
DO - 10.1016/j.fct.2010.09.013
M3 - Article
C2 - 20849907
AN - SCOPUS:78049448738
VL - 48
SP - 3412
EP - 3417
JO - Food and Chemical Toxicology
JF - Food and Chemical Toxicology
SN - 0278-6915
IS - 12
ER -