Potential use of quantum dots in flow cytometry

Raquel Ibáñez-Peral; Peter L. Bergquist; Malcolm R. Walter; Moreland Gibbs; Ewa M. Goldys; Belinda Ferrari

doi:10.3390/ijms9122622

Potential use of quantum dots in flow cytometry

Raquel Ibáñez-Peral^*, Peter L. Bergquist, Malcolm R. Walter, Moreland Gibbs, Ewa M. Goldys, Belinda Ferrari

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

27 Citations (Scopus)

Abstract

QDs may offer significant advantages in environmental and bead-based applications where the target cells need to be discriminated above background fluorescence. We have examined the possible applications of QDs for flow cytometric measurements (FCM) by studying their excitation - emission spectra and their binding to paramagnetic beads. We labelled beads with either QDs or a commonly-used fluorochrome (FITC) and studied their fluorescence intensity by FCM, Flow cytometric comparisons indicated that the minimum fluorophore concentration required for detection of QDs above autofluorescent background was 100-fold less than for FITC.

Original language	English
Pages (from-to)	2622-2638
Number of pages	17
Journal	International Journal of Molecular Sciences
Volume	9
Issue number	12
DOIs	https://doi.org/10.3390/ijms9122622
Publication status	Published - Dec 2008

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10.3390/ijms9122622

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abstract = "QDs may offer significant advantages in environmental and bead-based applications where the target cells need to be discriminated above background fluorescence. We have examined the possible applications of QDs for flow cytometric measurements (FCM) by studying their excitation - emission spectra and their binding to paramagnetic beads. We labelled beads with either QDs or a commonly-used fluorochrome (FITC) and studied their fluorescence intensity by FCM, Flow cytometric comparisons indicated that the minimum fluorophore concentration required for detection of QDs above autofluorescent background was 100-fold less than for FITC.",

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AU - Ibáñez-Peral, Raquel

AU - Bergquist, Peter L.

AU - Walter, Malcolm R.

AU - Gibbs, Moreland

AU - Goldys, Ewa M.

AU - Ferrari, Belinda

PY - 2008/12

Y1 - 2008/12

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AB - QDs may offer significant advantages in environmental and bead-based applications where the target cells need to be discriminated above background fluorescence. We have examined the possible applications of QDs for flow cytometric measurements (FCM) by studying their excitation - emission spectra and their binding to paramagnetic beads. We labelled beads with either QDs or a commonly-used fluorochrome (FITC) and studied their fluorescence intensity by FCM, Flow cytometric comparisons indicated that the minimum fluorophore concentration required for detection of QDs above autofluorescent background was 100-fold less than for FITC.

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Potential use of quantum dots in flow cytometry

Abstract

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