Potential use of quantum dots in flow cytometry

Raquel Ibáñez-Peral*, Peter L. Bergquist, Malcolm R. Walter, Moreland Gibbs, Ewa M. Goldys, Belinda Ferrari

*Corresponding author for this work

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

QDs may offer significant advantages in environmental and bead-based applications where the target cells need to be discriminated above background fluorescence. We have examined the possible applications of QDs for flow cytometric measurements (FCM) by studying their excitation - emission spectra and their binding to paramagnetic beads. We labelled beads with either QDs or a commonly-used fluorochrome (FITC) and studied their fluorescence intensity by FCM, Flow cytometric comparisons indicated that the minimum fluorophore concentration required for detection of QDs above autofluorescent background was 100-fold less than for FITC.

Original languageEnglish
Pages (from-to)2622-2638
Number of pages17
JournalInternational Journal of Molecular Sciences
Volume9
Issue number12
DOIs
Publication statusPublished - Dec 2008

Fingerprint Dive into the research topics of 'Potential use of quantum dots in flow cytometry'. Together they form a unique fingerprint.

Cite this