Practical implementation, characterization and applications of a multi-colour time-gated luminescence microscope

Lixin Zhang, Xianlin Zheng, Wei Deng, Yiqing Lu, Severine Lechevallier, Zhiqiang Ye, Ewa M. Goldys, Judith M. Dawes, James A. Piper, Jingli Yuan, Marc Verelst, Dayong Jin

Research output: Contribution to journalArticleResearchpeer-review

Abstract

Time-gated luminescence microscopy using long-lifetime molecular probes can effectively eliminate autofluorescence to enable high contrast imaging. Here we investigate a new strategy of time-gated imaging for simultaneous visualisation of multiple species of microorganisms stained with long-lived complexes under low-background conditions. This is realized by imaging two pathogenic organisms (Giardia lamblia stained with a red europium probe and Cryptosporidium parvum with a green terbium probe) at UV wavelengths (320-400 nm) through synchronization of a flash lamp with high repetition rate (1 kHz) to a robust time-gating detection unit. This approach provides four times enhancement in signal-to-background ratio over non-time-gated imaging, while the average signal intensity also increases six-fold compared with that under UV LED excitation. The high sensitivity is further confirmed by imaging the single europium-doped Y2O2S nanocrystals (150 nm). We report technical details regarding the time-gating detection unit and demonstrate its compatibility with commercial epi-fluorescence microscopes, providing a valuable and convenient addition to standard laboratory equipment.

LanguageEnglish
Article number6597
Pages1-6
Number of pages6
JournalScientific Reports
Volume4
DOIs
Publication statusPublished - 13 Oct 2014

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Luminescence
Color
Europium
Terbium
Cryptosporidium parvum
Molecular Probes
Giardia lamblia
Nanoparticles
Microscopy
Fluorescence
Equipment and Supplies

Bibliographical note

Copyright the Author(s) 2014. Version archived for private and non-commercial use with the permission of the author/s and according to publisher conditions. For further rights please contact the publisher.

Cite this

Zhang, Lixin ; Zheng, Xianlin ; Deng, Wei ; Lu, Yiqing ; Lechevallier, Severine ; Ye, Zhiqiang ; Goldys, Ewa M. ; Dawes, Judith M. ; Piper, James A. ; Yuan, Jingli ; Verelst, Marc ; Jin, Dayong. / Practical implementation, characterization and applications of a multi-colour time-gated luminescence microscope. In: Scientific Reports. 2014 ; Vol. 4. pp. 1-6.
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Practical implementation, characterization and applications of a multi-colour time-gated luminescence microscope. / Zhang, Lixin; Zheng, Xianlin; Deng, Wei; Lu, Yiqing; Lechevallier, Severine; Ye, Zhiqiang; Goldys, Ewa M.; Dawes, Judith M.; Piper, James A.; Yuan, Jingli; Verelst, Marc; Jin, Dayong.

In: Scientific Reports, Vol. 4, 6597, 13.10.2014, p. 1-6.

Research output: Contribution to journalArticleResearchpeer-review

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AU - Jin, Dayong

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