Preclinical 3D-model supports an invisibility cloak for adenoid cystic carcinoma

Rajdeep Chakraborty; Charbel Darido; Arthur Chien; Aidan Tay; Karen Vickery; Honghua Hu; Fei Liu; Shoba Ranganathan

doi:10.1038/s41598-023-44329-7

Preclinical 3D-model supports an invisibility cloak for adenoid cystic carcinoma

Rajdeep Chakraborty^*, Charbel Darido, Arthur Chien, Aidan Tay, Karen Vickery, Honghua Hu, Fei Liu, Shoba Ranganathan

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

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Abstract

The tumour-cell based initiation of immune evasion project evaluated the role of Gipie in adenoid cystic carcinoma (ACC) and mucoepidermoid carcinoma (A-253), from ninety-six 3D-ACC and A-253-immune co-culture models using natural killer cells (NK), and Jurkat cells (JK). Abnormal ACC morphology was observed in 3D-ACC immune co-culture models. Gipie-silencing conferred a “lymphoblast-like” morphology to ACC cells, a six-fold increase in apoptotic cells (compared to unaltered ACC cells, P ≤ 0.0001), a two-fold decrease in T regulatory cells (FoxP3⁺/IL-2Rα⁺/CD25⁺) (P ≤ 0.0001), and a three-fold increase in activated NK cells (NKp30⁺/IFN-γ⁺) (P ≤ 0.0001) with significantly higher release of granzyme (P ≤ 0.001) and perforin (P ≤ 0.0001).

Original language	English
Article number	17033
Pages (from-to)	1-7
Number of pages	7
Journal	Scientific Reports
Volume	13
DOIs	https://doi.org/10.1038/s41598-023-44329-7
Publication status	Published - 9 Oct 2023

Bibliographical note

Copyright the Author(s) 2023. Version archived for private and non-commercial use with the permission of the author/s and according to publisher conditions. For further rights please contact the publisher.

Keywords

Adenoid cystic carcinoma
Head and neck cancer
PROLIFERATION
Mucoepidermoid carcinoma
immune activation
Proteomic analysis
APOPTOSIS
3D cell culture
Immune evasion

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10.1038/s41598-023-44329-7Licence: CC BY

Publisher version (open access)Final published version, 1.69 MBLicence: CC BY

A tumour-cell based initiation of immune evasion
Chakraborty, R., Ranganathan, S. & Simpson, F.
1/10/21 → 30/09/22
Project: Research

Cite this

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title = "Preclinical 3D-model supports an invisibility cloak for adenoid cystic carcinoma",

abstract = "The tumour-cell based initiation of immune evasion project evaluated the role of Gipie in adenoid cystic carcinoma (ACC) and mucoepidermoid carcinoma (A-253), from ninety-six 3D-ACC and A-253-immune co-culture models using natural killer cells (NK), and Jurkat cells (JK). Abnormal ACC morphology was observed in 3D-ACC immune co-culture models. Gipie-silencing conferred a “lymphoblast-like” morphology to ACC cells, a six-fold increase in apoptotic cells (compared to unaltered ACC cells, P ≤ 0.0001), a two-fold decrease in T regulatory cells (FoxP3+/IL-2Rα+/CD25+) (P ≤ 0.0001), and a three-fold increase in activated NK cells (NKp30+/IFN-γ+) (P ≤ 0.0001) with significantly higher release of granzyme (P ≤ 0.001) and perforin (P ≤ 0.0001).",

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author = "Rajdeep Chakraborty and Charbel Darido and Arthur Chien and Aidan Tay and Karen Vickery and Honghua Hu and Fei Liu and Shoba Ranganathan",

note = "Copyright the Author(s) 2023. Version archived for private and non-commercial use with the permission of the author/s and according to publisher conditions. For further rights please contact the publisher.",

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doi = "10.1038/s41598-023-44329-7",

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AU - Chakraborty, Rajdeep

AU - Darido, Charbel

AU - Chien, Arthur

AU - Tay, Aidan

AU - Vickery, Karen

AU - Hu, Honghua

AU - Liu, Fei

AU - Ranganathan, Shoba

N1 - Copyright the Author(s) 2023. Version archived for private and non-commercial use with the permission of the author/s and according to publisher conditions. For further rights please contact the publisher.

PY - 2023/10/9

Y1 - 2023/10/9

N2 - The tumour-cell based initiation of immune evasion project evaluated the role of Gipie in adenoid cystic carcinoma (ACC) and mucoepidermoid carcinoma (A-253), from ninety-six 3D-ACC and A-253-immune co-culture models using natural killer cells (NK), and Jurkat cells (JK). Abnormal ACC morphology was observed in 3D-ACC immune co-culture models. Gipie-silencing conferred a “lymphoblast-like” morphology to ACC cells, a six-fold increase in apoptotic cells (compared to unaltered ACC cells, P ≤ 0.0001), a two-fold decrease in T regulatory cells (FoxP3+/IL-2Rα+/CD25+) (P ≤ 0.0001), and a three-fold increase in activated NK cells (NKp30+/IFN-γ+) (P ≤ 0.0001) with significantly higher release of granzyme (P ≤ 0.001) and perforin (P ≤ 0.0001).

AB - The tumour-cell based initiation of immune evasion project evaluated the role of Gipie in adenoid cystic carcinoma (ACC) and mucoepidermoid carcinoma (A-253), from ninety-six 3D-ACC and A-253-immune co-culture models using natural killer cells (NK), and Jurkat cells (JK). Abnormal ACC morphology was observed in 3D-ACC immune co-culture models. Gipie-silencing conferred a “lymphoblast-like” morphology to ACC cells, a six-fold increase in apoptotic cells (compared to unaltered ACC cells, P ≤ 0.0001), a two-fold decrease in T regulatory cells (FoxP3+/IL-2Rα+/CD25+) (P ≤ 0.0001), and a three-fold increase in activated NK cells (NKp30+/IFN-γ+) (P ≤ 0.0001) with significantly higher release of granzyme (P ≤ 0.001) and perforin (P ≤ 0.0001).

KW - Adenoid cystic carcinoma

KW - Head and neck cancer

KW - PROLIFERATION

KW - Mucoepidermoid carcinoma

KW - immune activation

KW - Proteomic analysis

KW - APOPTOSIS

KW - 3D cell culture

KW - Immune evasion

U2 - 10.1038/s41598-023-44329-7

DO - 10.1038/s41598-023-44329-7

M3 - Article

C2 - 37813936

AN - SCOPUS:85173650965

SN - 2045-2322

VL - 13

SP - 1

EP - 7

JO - Scientific Reports

JF - Scientific Reports

M1 - 17033

ER -

Preclinical 3D-model supports an invisibility cloak for adenoid cystic carcinoma

Abstract

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Keywords

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A tumour-cell based initiation of immune evasion

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