TY - JOUR
T1 - Preparation and time-resolved luminescence bioassay application of multicolor luminescent lanthanide nanoparticles
AU - Jiang, Hongfei
AU - Wang, Guilan
AU - Zhang, Wenzhu
AU - Liu, Xiaoyu
AU - Ye, Zhiqiang
AU - Jin, Dayong
AU - Yuan, Jingli
AU - Liu, Zhiguang
PY - 2010/1
Y1 - 2010/1
N2 - Because highly luminescent lanthanide compounds are limited to Eu 3+ and Tb 3+ compounds with red (Eu, ~615 nm) and green (Tb, ~545 nm) emission colors, the development and application of time-resolved luminescence bioassay technique using lanthanide-based multicolor luminescent biolabels have rarely been investigated. In this work, a series of lanthanide complexes covalently bound silica nanoparticles with an excitation maximum wavelength at 335 nm and red, orange, yellow and green emission colors has been prepared by co-binding different molar ratios of luminescent Eu 3+-Tb 3+ complexes with a ligand ,N,N 1, 1-(4′-phenyl-2, ′:6′, 2′-terpyridine-6, 6′-diyl)bis(methylenenitrilo) tetrakis (acetic acid) inside the silica nanoparticles. The nanoparticles characterized by transmission electron microscopy and luminescence spectroscopy methods were used for streptavidin labeling, and time-resolved fluoroimmunoassay (TR-FIA) of human prostate-specific antigen (PSA) as well as time-resolved luminescence imaging detection of an environmental pathogen, Giardia lamblia. The results demonstrated the utility of the new multicolor luminescent lanthanide nanoparticles for time-resolved luminescence bioassays.
AB - Because highly luminescent lanthanide compounds are limited to Eu 3+ and Tb 3+ compounds with red (Eu, ~615 nm) and green (Tb, ~545 nm) emission colors, the development and application of time-resolved luminescence bioassay technique using lanthanide-based multicolor luminescent biolabels have rarely been investigated. In this work, a series of lanthanide complexes covalently bound silica nanoparticles with an excitation maximum wavelength at 335 nm and red, orange, yellow and green emission colors has been prepared by co-binding different molar ratios of luminescent Eu 3+-Tb 3+ complexes with a ligand ,N,N 1, 1-(4′-phenyl-2, ′:6′, 2′-terpyridine-6, 6′-diyl)bis(methylenenitrilo) tetrakis (acetic acid) inside the silica nanoparticles. The nanoparticles characterized by transmission electron microscopy and luminescence spectroscopy methods were used for streptavidin labeling, and time-resolved fluoroimmunoassay (TR-FIA) of human prostate-specific antigen (PSA) as well as time-resolved luminescence imaging detection of an environmental pathogen, Giardia lamblia. The results demonstrated the utility of the new multicolor luminescent lanthanide nanoparticles for time-resolved luminescence bioassays.
UR - http://www.scopus.com/inward/record.url?scp=77951025280&partnerID=8YFLogxK
U2 - 10.1007/s10895-009-0559-7
DO - 10.1007/s10895-009-0559-7
M3 - Article
C2 - 19851848
AN - SCOPUS:77951025280
VL - 20
SP - 321
EP - 328
JO - Journal of Fluorescence
JF - Journal of Fluorescence
SN - 1053-0509
IS - 1
ER -