Preparation and time-resolved luminescence bioassay application of multicolor luminescent lanthanide nanoparticles

Hongfei Jiang, Guilan Wang, Wenzhu Zhang, Xiaoyu Liu, Zhiqiang Ye*, Dayong Jin, Jingli Yuan, Zhiguang Liu

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

52 Citations (Scopus)


Because highly luminescent lanthanide compounds are limited to Eu 3+ and Tb 3+ compounds with red (Eu, ~615 nm) and green (Tb, ~545 nm) emission colors, the development and application of time-resolved luminescence bioassay technique using lanthanide-based multicolor luminescent biolabels have rarely been investigated. In this work, a series of lanthanide complexes covalently bound silica nanoparticles with an excitation maximum wavelength at 335 nm and red, orange, yellow and green emission colors has been prepared by co-binding different molar ratios of luminescent Eu 3+-Tb 3+ complexes with a ligand ,N,N 1, 1-(4′-phenyl-2, ′:6′, 2′-terpyridine-6, 6′-diyl)bis(methylenenitrilo) tetrakis (acetic acid) inside the silica nanoparticles. The nanoparticles characterized by transmission electron microscopy and luminescence spectroscopy methods were used for streptavidin labeling, and time-resolved fluoroimmunoassay (TR-FIA) of human prostate-specific antigen (PSA) as well as time-resolved luminescence imaging detection of an environmental pathogen, Giardia lamblia. The results demonstrated the utility of the new multicolor luminescent lanthanide nanoparticles for time-resolved luminescence bioassays.

Original languageEnglish
Pages (from-to)321-328
Number of pages8
JournalJournal of Fluorescence
Issue number1
Publication statusPublished - Jan 2010


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