Prevalence and molecular identification of nematode and dipteran parasites in an Australian alpine grasshopper (Kosciuscola tristis)

Kate D L Umbers, Lachlan J. Byatt, Nichola J. Hill, Remo J. Bartolini, Grant C. Hose, Marie E. Herberstein, Michelle L. Power

Research output: Contribution to journalArticleResearchpeer-review

Abstract

In alpine Australia, Orthoptera are abundant, dominant herbivores, important prey species, and hosts for parasites and parasitoids. Despite the central role of orthopterans in alpine ecosystems, the impact of parasites on orthopteran populations is under-explored. In this study we describe the relationship between parasite prevalence and host sex, body size and year of collection. We accessed an existing, preserved collection of 640 Kosciuscola tristis collected from across its range between 2007 and 2011. Upon dissection we collected juvenile parasites and used molecular tools to identify them to three families (Nematoda; Mermithidae, and Arthropoda: Diptera: Tachinidae and Sarcophagidae). The prevalence of nematodes ranged from 3.5% to 25.0% and dipterans from 2.4% to 20.0%. Contrary to predictions, we found no associations between parasite prevalence and grasshopper sex or size. Although there was an association between prevalence of both nematodes and dipterans with year of collection, this is likely driven by a small sample size in the first year. Our results provide a foundation for future studies into parasite prevalence within the alpine environment and the abiotic factors that might influence these associations.

LanguageEnglish
Article numbere0121685
Pages1-11
Number of pages11
JournalPLoS ONE
Volume10
Issue number4
DOIs
Publication statusPublished - 28 Apr 2015

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Grasshoppers
grasshoppers
Parasites
Nematoda
parasites
Orthoptera
Sarcophagidae
Mermithidae
Dissection
Arthropoda
Tachinidae
Herbivory
Arthropods
gender
Body Size
Diptera
Sample Size
Ecosystems
parasitoids
Ecosystem

Bibliographical note

Copyright the Author(s) 2015. Version archived for private and non-commercial use with the permission of the author/s and according to publisher conditions. For further rights please contact the publisher.

Cite this

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title = "Prevalence and molecular identification of nematode and dipteran parasites in an Australian alpine grasshopper (Kosciuscola tristis)",
abstract = "In alpine Australia, Orthoptera are abundant, dominant herbivores, important prey species, and hosts for parasites and parasitoids. Despite the central role of orthopterans in alpine ecosystems, the impact of parasites on orthopteran populations is under-explored. In this study we describe the relationship between parasite prevalence and host sex, body size and year of collection. We accessed an existing, preserved collection of 640 Kosciuscola tristis collected from across its range between 2007 and 2011. Upon dissection we collected juvenile parasites and used molecular tools to identify them to three families (Nematoda; Mermithidae, and Arthropoda: Diptera: Tachinidae and Sarcophagidae). The prevalence of nematodes ranged from 3.5{\%} to 25.0{\%} and dipterans from 2.4{\%} to 20.0{\%}. Contrary to predictions, we found no associations between parasite prevalence and grasshopper sex or size. Although there was an association between prevalence of both nematodes and dipterans with year of collection, this is likely driven by a small sample size in the first year. Our results provide a foundation for future studies into parasite prevalence within the alpine environment and the abiotic factors that might influence these associations.",
author = "Umbers, {Kate D L} and Byatt, {Lachlan J.} and Hill, {Nichola J.} and Bartolini, {Remo J.} and Hose, {Grant C.} and Herberstein, {Marie E.} and Power, {Michelle L.}",
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Prevalence and molecular identification of nematode and dipteran parasites in an Australian alpine grasshopper (Kosciuscola tristis). / Umbers, Kate D L; Byatt, Lachlan J.; Hill, Nichola J.; Bartolini, Remo J.; Hose, Grant C.; Herberstein, Marie E.; Power, Michelle L.

In: PLoS ONE, Vol. 10, No. 4, e0121685, 28.04.2015, p. 1-11.

Research output: Contribution to journalArticleResearchpeer-review

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