Primary air-liquid interface culture of nasal epithelium for nasal drug delivery

Hui Xin Ong*, Claire L. Jackson, Janice L. Cole, Peter M. Lackie, Daniela Traini, Paul M. Young, Jane Lucas, Joy Conway

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

21 Citations (Scopus)

Abstract

Nasal drug administration is a promising alternative to oral and parenteral administration for both local and systemic delivery of drugs. The benefits include its noninvasive nature, rapid absorption, and circumvention of first pass metabolism. Hence, the use of an in vitro model using human primary nasal epithelial cells could be key to understanding important functions and parameters of the respiratory epithelium. This model will enable investigators to address important and original research questions using a biologically relevant in vitro platform that mimics the in vivo nasal epithelial physiology. The purpose of this study was to establish, systematically characterize, and validate the use of a primary human nasal epithelium model cultured at the air-liquid interface for the study of inflammatory responses and drug transport and to simultaneously quantify drug effects on ciliary activity.

Original languageEnglish
Pages (from-to)2242-2252
Number of pages11
JournalMolecular Pharmaceutics
Volume13
Issue number7
DOIs
Publication statusPublished - 5 Jul 2016
Externally publishedYes

Keywords

  • ciliary function
  • epithelial cells
  • nasal drug delivery
  • tobramycin
  • transport

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