Primary structure of a Plasmodium falciparum rhoptry antigen

Heidi J. Brown; Ross L. Coppel

doi:10.1016/0166-6851(91)90133-Q

Primary structure of a Plasmodium falciparum rhoptry antigen

Heidi J. Brown, Ross L. Coppel^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

69 Citations (Scopus)

Abstract

The high-molecular-weight rhoptry complex of Plasmodium falciparum consists of 3 non-covalently associated polypeptides of 150, 135 and 105 kDa. We present the complete nucleotide sequence of the 105-kDa (RhopH3) component of this complex derived from analysis of genomic and cDNA clones. The genomic structure is unusually complex for P. falciparum, consisting of 7 exons including 2 mini-exons of 19 and 21 amino acids. The sequence lacks tandem repeats and is conserved among several parasite isolates. B cell epitopes that induce antibody responses during natural infection were mapped to five different regions of the polypeptide.

Original language	English
Pages (from-to)	99-110
Number of pages	12
Journal	Molecular and Biochemical Parasitology
Volume	49
Issue number	1
DOIs	https://doi.org/10.1016/0166-6851(91)90133-Q
Publication status	Published - Nov 1991
Externally published	Yes

Keywords

Plasmodium falciparum
rhoptry antigen
intron
RhopH3

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10.1016/0166-6851(91)90133-Q

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AU - Coppel, Ross L.

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AB - The high-molecular-weight rhoptry complex of Plasmodium falciparum consists of 3 non-covalently associated polypeptides of 150, 135 and 105 kDa. We present the complete nucleotide sequence of the 105-kDa (RhopH3) component of this complex derived from analysis of genomic and cDNA clones. The genomic structure is unusually complex for P. falciparum, consisting of 7 exons including 2 mini-exons of 19 and 21 amino acids. The sequence lacks tandem repeats and is conserved among several parasite isolates. B cell epitopes that induce antibody responses during natural infection were mapped to five different regions of the polypeptide.

KW - Plasmodium falciparum

KW - rhoptry antigen

KW - intron

KW - RhopH3

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VL - 49

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EP - 110

JO - Molecular and Biochemical Parasitology

JF - Molecular and Biochemical Parasitology

IS - 1

ER -

Primary structure of a Plasmodium falciparum rhoptry antigen

Abstract

Keywords

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