Primed for success

oyster parents treated with poly(I:C) produce offspring with enhanced protection against Ostreid herpesvirus type I infection

Timothy J. Green*, Karla Helbig, Peter Speck, David A. Raftos

*Corresponding author for this work

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

The Pacific oyster (Crassostrea gigas) is farmed globally. Ostreid herpesvirus (OsHV-1) causes severe mortalities of farmed C. gigas. Management of OsHV-1 has proven difficult. Oysters treated with poly(I:C) exhibit enhanced protection (EP) against OsHV-1. This chemical treatment is highly effective, but it is not feasible to treat every oyster on a farm. To circumvent this practical limitation, previous studies on arthropods have suggested that EP can be transferred from parents to their offspring (trans-generational EP, TGEP). This suggests that the treatment of relatively few parents could be used to produce large numbers of offspring with TGEP. Here, we investigated TGEP in oysters to test whether it might be used as a cost effective management tool to control OsHV-1. We found that offspring (D-veliger larvae) produced from poly(I:C)-treated parents had double the chance of surviving exposure to OsHV-1 compared to controls. Furthermore, the larvae of poly(I:C)-treated parents contained elevated levels of mRNA encoding a key transcription factor that regulates antiviral immunity (IRF2). Poly(I:C) treatment had no effect on the survival of oyster parents. Hence, the enhanced immunity of their offspring could not be explained by genetic selection, and instead may reflect epigenetic reprogramming or maternal provisioning.

Original languageEnglish
Pages (from-to)113-120
Number of pages8
JournalMolecular Immunology
Volume78
DOIs
Publication statusPublished - 1 Oct 2016

Keywords

  • Crassostrea
  • OsHV-1
  • trans-generational immune priming
  • trained immunity
  • invertebrate antiviral response

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