Production of recombinant bleaching enzymes from thermophilic microorganisms in fungal hosts

Peter L. Bergquist; V. S. Junior Te’o; Moreland D. Gibbs; Angela C.E. Cziferszky; Fabricia P. De Faria; Maristela O. Azevedo; K. M.Helena Nevalainen

doi:10.1385/ABAB:98-100:1-9:1651

Production of recombinant bleaching enzymes from thermophilic microorganisms in fungal hosts

Peter L. Bergquist^*, V. S. Junior Te’o, Moreland D. Gibbs, Angela C.E. Cziferszky, Fabricia P. De Faria, Maristela O. Azevedo, K. M.Helena Nevalainen

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

14 Citations (Scopus)

Abstract

Cost-effective production of enzymes for industrial processes makes the appropriate selection of the host-vector expression system critical. We have developed two systems for the bulk production of bleaching enzymes from thermophiles. Kluyveromyces lactis has been developed as a secretion host employing expression vectors based on the 2μ-like plasmid pKD1 of Kluyveromyces drosophilarium. Our second system involves the filamentous fungus Trichoderma reesei. Fusion and nonfusion vectors have been constructed using the strong cellobiohydrolase 1 (cbh1) promoter. The KEX2 protease cleavage site and a 6 × HIS-tag have been incorporated to facilitate both cleavage and purification of the mature foreign proteins.

Original language	English
Pages (from-to)	165-176
Number of pages	12
Journal	Applied Biochemistry and Biotechnology
Volume	98-100
Issue number	1-9
DOIs	https://doi.org/10.1385/ABAB:98-100:1-9:1651
Publication status	Published - 2002

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abstract = "Cost-effective production of enzymes for industrial processes makes the appropriate selection of the host-vector expression system critical. We have developed two systems for the bulk production of bleaching enzymes from thermophiles. Kluyveromyces lactis has been developed as a secretion host employing expression vectors based on the 2μ-like plasmid pKD1 of Kluyveromyces drosophilarium. Our second system involves the filamentous fungus Trichoderma reesei. Fusion and nonfusion vectors have been constructed using the strong cellobiohydrolase 1 (cbh1) promoter. The KEX2 protease cleavage site and a 6 × HIS-tag have been incorporated to facilitate both cleavage and purification of the mature foreign proteins.",

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AU - Bergquist, Peter L.

AU - Te’o, V. S. Junior

AU - Gibbs, Moreland D.

AU - Cziferszky, Angela C.E.

AU - De Faria, Fabricia P.

AU - Azevedo, Maristela O.

AU - Nevalainen, K. M.Helena

PY - 2002

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AB - Cost-effective production of enzymes for industrial processes makes the appropriate selection of the host-vector expression system critical. We have developed two systems for the bulk production of bleaching enzymes from thermophiles. Kluyveromyces lactis has been developed as a secretion host employing expression vectors based on the 2μ-like plasmid pKD1 of Kluyveromyces drosophilarium. Our second system involves the filamentous fungus Trichoderma reesei. Fusion and nonfusion vectors have been constructed using the strong cellobiohydrolase 1 (cbh1) promoter. The KEX2 protease cleavage site and a 6 × HIS-tag have been incorporated to facilitate both cleavage and purification of the mature foreign proteins.

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Production of recombinant bleaching enzymes from thermophilic microorganisms in fungal hosts

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