Programmable LED-based integrating sphere light source for wide-field fluorescence microscopy

Aziz ul Rehman; Ayad G. Anwer; Ewa M. Goldys

doi:10.1016/j.pdpdt.2017.10.002

Programmable LED-based integrating sphere light source for wide-field fluorescence microscopy

Aziz ul Rehman^*, Ayad G. Anwer, Ewa M. Goldys

^*Corresponding author for this work

ARC Centre of Excellence for Nanoscale BioPhotonics (CNBP)

Research output: Contribution to journal › Article › peer-review

12 Citations (Scopus)

Abstract

Wide-field fluorescence microscopy commonly uses a mercury lamp, which has limited spectral capabilities. We designed and built a programmable integrating sphere light (PISL) source which consists of nine LEDs, light-collecting optics, a commercially available integrating sphere and a baffle. The PISL source is tuneable in the range 365–490 nm with a uniform spatial profile and a sufficient power at the objective to carry out spectral imaging. We retrofitted a standard fluorescence inverted microscope DM IRB (Leica) with a PISL source by mounting it together with a highly sensitive low- noise CMOS camera. The capabilities of the setup have been demonstrated by carrying out multispectral autofluorescence imaging of live BV2 cells.

Original language	English
Pages (from-to)	201-206
Number of pages	6
Journal	Photodiagnosis and Photodynamic Therapy
Volume	20
DOIs	https://doi.org/10.1016/j.pdpdt.2017.10.002
Publication status	Published - 1 Dec 2017

Keywords

integrating sphere
fluorescence microscopy
BV2Cells
spectral spatial profile
low noise CMOS camera

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10.1016/j.pdpdt.2017.10.002

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title = "Programmable LED-based integrating sphere light source for wide-field fluorescence microscopy",

abstract = "Wide-field fluorescence microscopy commonly uses a mercury lamp, which has limited spectral capabilities. We designed and built a programmable integrating sphere light (PISL) source which consists of nine LEDs, light-collecting optics, a commercially available integrating sphere and a baffle. The PISL source is tuneable in the range 365–490 nm with a uniform spatial profile and a sufficient power at the objective to carry out spectral imaging. We retrofitted a standard fluorescence inverted microscope DM IRB (Leica) with a PISL source by mounting it together with a highly sensitive low- noise CMOS camera. The capabilities of the setup have been demonstrated by carrying out multispectral autofluorescence imaging of live BV2 cells.",

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AU - Anwer, Ayad G.

AU - Goldys, Ewa M.

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AB - Wide-field fluorescence microscopy commonly uses a mercury lamp, which has limited spectral capabilities. We designed and built a programmable integrating sphere light (PISL) source which consists of nine LEDs, light-collecting optics, a commercially available integrating sphere and a baffle. The PISL source is tuneable in the range 365–490 nm with a uniform spatial profile and a sufficient power at the objective to carry out spectral imaging. We retrofitted a standard fluorescence inverted microscope DM IRB (Leica) with a PISL source by mounting it together with a highly sensitive low- noise CMOS camera. The capabilities of the setup have been demonstrated by carrying out multispectral autofluorescence imaging of live BV2 cells.

KW - integrating sphere

KW - fluorescence microscopy

KW - BV2Cells

KW - spectral spatial profile

KW - low noise CMOS camera

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Programmable LED-based integrating sphere light source for wide-field fluorescence microscopy

Abstract

Keywords

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