Programmable LED-based integrating sphere light source for wide-field fluorescence microscopy

Aziz ul Rehman*, Ayad G. Anwer, Ewa M. Goldys

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

8 Citations (Scopus)

Abstract

Wide-field fluorescence microscopy commonly uses a mercury lamp, which has limited spectral capabilities. We designed and built a programmable integrating sphere light (PISL) source which consists of nine LEDs, light-collecting optics, a commercially available integrating sphere and a baffle. The PISL source is tuneable in the range 365–490 nm with a uniform spatial profile and a sufficient power at the objective to carry out spectral imaging. We retrofitted a standard fluorescence inverted microscope DM IRB (Leica) with a PISL source by mounting it together with a highly sensitive low- noise CMOS camera. The capabilities of the setup have been demonstrated by carrying out multispectral autofluorescence imaging of live BV2 cells.

Original languageEnglish
Pages (from-to)201-206
Number of pages6
JournalPhotodiagnosis and Photodynamic Therapy
Volume20
DOIs
Publication statusPublished - 1 Dec 2017

Keywords

  • integrating sphere
  • fluorescence microscopy
  • BV2Cells
  • spectral spatial profile
  • low noise CMOS camera

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