Programmable polyketide biosynthesis platform for production of aromatic compounds in yeast

Tadas Jakočiūnas; Andreas K. Klitgaard; Eftychia Eva Kontou; Julie Bang Nielsen; Emil Thomsen; David Romero-Suarez; Kai Blin; Christopher J. Petzold; Jennifer W. Gin; Yaojun Tong; Charlotte Held Gotfredsen; Pep Charusanti; Rasmus J.N. Frandsen; Tilmann Weber; Sang Yup Lee; Michael K. Jensen; Jay D. Keasling

doi:10.1016/j.synbio.2020.01.004

Programmable polyketide biosynthesis platform for production of aromatic compounds in yeast

Tadas Jakočiūnas^*, Andreas K. Klitgaard, Eftychia Eva Kontou, Julie Bang Nielsen, Emil Thomsen, David Romero-Suarez, Kai Blin, Christopher J. Petzold, Jennifer W. Gin, Yaojun Tong, Charlotte Held Gotfredsen, Pep Charusanti, Rasmus J.N. Frandsen, Tilmann Weber, Sang Yup Lee, Michael K. Jensen, Jay D. Keasling^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

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Abstract

To accelerate the shift to bio-based production and overcome complicated functional implementation of natural and artificial biosynthetic pathways to industry relevant organisms, development of new, versatile, bio-based production platforms is required. Here we present a novel yeast-based platform for biosynthesis of bacterial aromatic polyketides. The platform is based on a synthetic polyketide synthase system enabling a first demonstration of bacterial aromatic polyketide biosynthesis in a eukaryotic host.

Original language	English
Pages (from-to)	11-18
Number of pages	8
Journal	Synthetic and Systems Biotechnology
Volume	5
Issue number	1
Early online date	23 Jan 2020
DOIs	https://doi.org/10.1016/j.synbio.2020.01.004
Publication status	Published - Mar 2020
Externally published	Yes

Bibliographical note

Version archived for private and non-commercial use with the permission of the author/s and according to publisher conditions. For further rights please contact the publisher.

Keywords

Aromatic polyketides
Biotechnology
CRISPR/Cas9
Metabolic engineering
Natural products
Polyketide
Production platform in eukaryotes
Synthetic biology

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10.1016/j.synbio.2020.01.004

Publisher version (open access)Final published version, 1.87 MBLicence: CC BY

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Jakočiūnas, T., Klitgaard, A. K., Kontou, E. E., Nielsen, J. B., Thomsen, E., Romero-Suarez, D., Blin, K., Petzold, C. J., Gin, J. W., Tong, Y., Gotfredsen, C. H., Charusanti, P., Frandsen, R. J. N., Weber, T., Lee, S. Y., Jensen, M. K., & Keasling, J. D. (2020). Programmable polyketide biosynthesis platform for production of aromatic compounds in yeast. Synthetic and Systems Biotechnology, 5(1), 11-18. https://doi.org/10.1016/j.synbio.2020.01.004

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title = "Programmable polyketide biosynthesis platform for production of aromatic compounds in yeast",

abstract = "To accelerate the shift to bio-based production and overcome complicated functional implementation of natural and artificial biosynthetic pathways to industry relevant organisms, development of new, versatile, bio-based production platforms is required. Here we present a novel yeast-based platform for biosynthesis of bacterial aromatic polyketides. The platform is based on a synthetic polyketide synthase system enabling a first demonstration of bacterial aromatic polyketide biosynthesis in a eukaryotic host.",

keywords = "Aromatic polyketides, Biotechnology, CRISPR/Cas9, Metabolic engineering, Natural products, Polyketide, Production platform in eukaryotes, Synthetic biology",

author = "Tadas Jako{\v c}iūnas and Klitgaard, {Andreas K.} and Kontou, {Eftychia Eva} and Nielsen, {Julie Bang} and Emil Thomsen and David Romero-Suarez and Kai Blin and Petzold, {Christopher J.} and Gin, {Jennifer W.} and Yaojun Tong and Gotfredsen, {Charlotte Held} and Pep Charusanti and Frandsen, {Rasmus J.N.} and Tilmann Weber and Lee, {Sang Yup} and Jensen, {Michael K.} and Keasling, {Jay D.}",

note = "Version archived for private and non-commercial use with the permission of the author/s and according to publisher conditions. For further rights please contact the publisher. ",

year = "2020",

month = mar,

doi = "10.1016/j.synbio.2020.01.004",

language = "English",

volume = "5",

pages = "11--18",

journal = "Synthetic and Systems Biotechnology",

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Jakočiūnas, T, Klitgaard, AK, Kontou, EE, Nielsen, JB, Thomsen, E, Romero-Suarez, D, Blin, K, Petzold, CJ, Gin, JW, Tong, Y, Gotfredsen, CH, Charusanti, P, Frandsen, RJN, Weber, T, Lee, SY, Jensen, MK & Keasling, JD 2020, 'Programmable polyketide biosynthesis platform for production of aromatic compounds in yeast', Synthetic and Systems Biotechnology, vol. 5, no. 1, pp. 11-18. https://doi.org/10.1016/j.synbio.2020.01.004

TY - JOUR

T1 - Programmable polyketide biosynthesis platform for production of aromatic compounds in yeast

AU - Jakočiūnas, Tadas

AU - Klitgaard, Andreas K.

AU - Kontou, Eftychia Eva

AU - Nielsen, Julie Bang

AU - Thomsen, Emil

AU - Romero-Suarez, David

AU - Blin, Kai

AU - Petzold, Christopher J.

AU - Gin, Jennifer W.

AU - Tong, Yaojun

AU - Gotfredsen, Charlotte Held

AU - Charusanti, Pep

AU - Frandsen, Rasmus J.N.

AU - Weber, Tilmann

AU - Lee, Sang Yup

AU - Jensen, Michael K.

AU - Keasling, Jay D.

N1 - Version archived for private and non-commercial use with the permission of the author/s and according to publisher conditions. For further rights please contact the publisher.

PY - 2020/3

Y1 - 2020/3

N2 - To accelerate the shift to bio-based production and overcome complicated functional implementation of natural and artificial biosynthetic pathways to industry relevant organisms, development of new, versatile, bio-based production platforms is required. Here we present a novel yeast-based platform for biosynthesis of bacterial aromatic polyketides. The platform is based on a synthetic polyketide synthase system enabling a first demonstration of bacterial aromatic polyketide biosynthesis in a eukaryotic host.

AB - To accelerate the shift to bio-based production and overcome complicated functional implementation of natural and artificial biosynthetic pathways to industry relevant organisms, development of new, versatile, bio-based production platforms is required. Here we present a novel yeast-based platform for biosynthesis of bacterial aromatic polyketides. The platform is based on a synthetic polyketide synthase system enabling a first demonstration of bacterial aromatic polyketide biosynthesis in a eukaryotic host.

KW - Aromatic polyketides

KW - Biotechnology

KW - CRISPR/Cas9

KW - Metabolic engineering

KW - Natural products

KW - Polyketide

KW - Production platform in eukaryotes

KW - Synthetic biology

UR - http://www.scopus.com/inward/record.url?scp=85078510384&partnerID=8YFLogxK

U2 - 10.1016/j.synbio.2020.01.004

DO - 10.1016/j.synbio.2020.01.004

M3 - Article

C2 - 32021916

SN - 2405-805X

VL - 5

SP - 11

EP - 18

JO - Synthetic and Systems Biotechnology

JF - Synthetic and Systems Biotechnology

IS - 1

ER -

Programmable polyketide biosynthesis platform for production of aromatic compounds in yeast

Abstract

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