Prospecting for novel lipase genes using PCR

Philip J. L. Bell, Anwar Sunna, Moreland D. Gibbs, Natalie C. Curach, Helena Nevalainen, Peter L. Bergquist

Research output: Contribution to journalArticleResearchpeer-review

Abstract

A PCR method suitable for the isolation of lipase genes directly from environmental DNA is described. The problems associated with the low levels of similarity between lipase genes were overcome by extensive analysis of conserved regions and careful primer design. Using this method, a lipase gene (oli-lipase) was isolated directly from environmental DNA. This lipase showed less than 20% similarity with other known lipases at the amino acid level. The study also revealed that distantly related members of the α/β hydrolase superfamily share similar conserved motifs with the lipases, thus making these genes targets for gene prospecting by PCR.

LanguageEnglish
Pages2283-2291
Number of pages9
JournalMicrobiology
Volume148
Issue number8
DOIs
Publication statusPublished - Aug 2002

Fingerprint

Lipase
Polymerase Chain Reaction
Genes
DNA
Hydrolases
Amino Acids

Keywords

  • thermophilic
  • triacylglycerol hydrolase
  • biomass
  • environment

Cite this

Bell, P. J. L., Sunna, A., Gibbs, M. D., Curach, N. C., Nevalainen, H., & Bergquist, P. L. (2002). Prospecting for novel lipase genes using PCR. Microbiology, 148(8), 2283-2291. https://doi.org/10.1099/00221287-148-8-2283
Bell, Philip J. L. ; Sunna, Anwar ; Gibbs, Moreland D. ; Curach, Natalie C. ; Nevalainen, Helena ; Bergquist, Peter L. / Prospecting for novel lipase genes using PCR. In: Microbiology. 2002 ; Vol. 148, No. 8. pp. 2283-2291.
@article{8ce267857bea4a318e56f4ed5329870a,
title = "Prospecting for novel lipase genes using PCR",
abstract = "A PCR method suitable for the isolation of lipase genes directly from environmental DNA is described. The problems associated with the low levels of similarity between lipase genes were overcome by extensive analysis of conserved regions and careful primer design. Using this method, a lipase gene (oli-lipase) was isolated directly from environmental DNA. This lipase showed less than 20{\%} similarity with other known lipases at the amino acid level. The study also revealed that distantly related members of the α/β hydrolase superfamily share similar conserved motifs with the lipases, thus making these genes targets for gene prospecting by PCR.",
keywords = "thermophilic, triacylglycerol hydrolase, biomass, environment",
author = "Bell, {Philip J. L.} and Anwar Sunna and Gibbs, {Moreland D.} and Curach, {Natalie C.} and Helena Nevalainen and Bergquist, {Peter L.}",
year = "2002",
month = "8",
doi = "10.1099/00221287-148-8-2283",
language = "English",
volume = "148",
pages = "2283--2291",
journal = "Microbiology",
issn = "1350-0872",
publisher = "Microbiology Society",
number = "8",

}

Bell, PJL, Sunna, A, Gibbs, MD, Curach, NC, Nevalainen, H & Bergquist, PL 2002, 'Prospecting for novel lipase genes using PCR', Microbiology, vol. 148, no. 8, pp. 2283-2291. https://doi.org/10.1099/00221287-148-8-2283

Prospecting for novel lipase genes using PCR. / Bell, Philip J. L.; Sunna, Anwar; Gibbs, Moreland D.; Curach, Natalie C.; Nevalainen, Helena; Bergquist, Peter L.

In: Microbiology, Vol. 148, No. 8, 08.2002, p. 2283-2291.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - Prospecting for novel lipase genes using PCR

AU - Bell, Philip J. L.

AU - Sunna, Anwar

AU - Gibbs, Moreland D.

AU - Curach, Natalie C.

AU - Nevalainen, Helena

AU - Bergquist, Peter L.

PY - 2002/8

Y1 - 2002/8

N2 - A PCR method suitable for the isolation of lipase genes directly from environmental DNA is described. The problems associated with the low levels of similarity between lipase genes were overcome by extensive analysis of conserved regions and careful primer design. Using this method, a lipase gene (oli-lipase) was isolated directly from environmental DNA. This lipase showed less than 20% similarity with other known lipases at the amino acid level. The study also revealed that distantly related members of the α/β hydrolase superfamily share similar conserved motifs with the lipases, thus making these genes targets for gene prospecting by PCR.

AB - A PCR method suitable for the isolation of lipase genes directly from environmental DNA is described. The problems associated with the low levels of similarity between lipase genes were overcome by extensive analysis of conserved regions and careful primer design. Using this method, a lipase gene (oli-lipase) was isolated directly from environmental DNA. This lipase showed less than 20% similarity with other known lipases at the amino acid level. The study also revealed that distantly related members of the α/β hydrolase superfamily share similar conserved motifs with the lipases, thus making these genes targets for gene prospecting by PCR.

KW - thermophilic

KW - triacylglycerol hydrolase

KW - biomass

KW - environment

UR - http://www.scopus.com/inward/record.url?scp=0036667416&partnerID=8YFLogxK

U2 - 10.1099/00221287-148-8-2283

DO - 10.1099/00221287-148-8-2283

M3 - Article

VL - 148

SP - 2283

EP - 2291

JO - Microbiology

T2 - Microbiology

JF - Microbiology

SN - 1350-0872

IS - 8

ER -