Prostaglandin E2 elevates calcium in differentiated neuroectodermal stem cells

Jennilee M. Davidson; Christine T. Wong; Ravneet Rai-Bhogal; Hongyan Li; Dorota A. Crawford

doi:10.1016/j.mcn.2016.03.010

Prostaglandin E2 elevates calcium in differentiated neuroectodermal stem cells

Jennilee M. Davidson, Christine T. Wong, Ravneet Rai-Bhogal, Hongyan Li, Dorota A. Crawford^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

10 Citations (Scopus)

Abstract

Lipid mediator prostaglandin E₂ (PGE₂) is an endogenous signaling molecule that plays an important role during early development of the nervous system. Abnormalities in the PGE₂ signaling pathway have been associated with neurodevelopmental disorders such as autism spectrum disorders. In this study we use ratiometric fura-2AM calcium imaging to show that higher levels of PGE₂ elevate intracellular calcium levels in the cell soma and growth cones of differentiated neuroectodermal (NE-4C) stem cells. PGE₂ also increased the amplitude of calcium fluctuation in the neuronal growth cones and affected the neurite extension length. In summary, our results show that PGE₂ may adversely impact intracellular calcium dynamics in differentiated neuronal cells and possibly affect early development of the nervous system.

Original language	English
Pages (from-to)	71-77
Number of pages	7
Journal	Molecular and Cellular Neuroscience
Volume	74
DOIs	https://doi.org/10.1016/j.mcn.2016.03.010
Publication status	Published - 1 Jul 2016
Externally published	Yes

Keywords

Autism spectrum disorders
Calcium fluctuations
Differentiated neuroectodermal stem cells
Growth cone
Neurite extension
Neurons
Prostaglandin E2

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10.1016/j.mcn.2016.03.010

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title = "Prostaglandin E2 elevates calcium in differentiated neuroectodermal stem cells",

abstract = "Lipid mediator prostaglandin E2 (PGE2) is an endogenous signaling molecule that plays an important role during early development of the nervous system. Abnormalities in the PGE2 signaling pathway have been associated with neurodevelopmental disorders such as autism spectrum disorders. In this study we use ratiometric fura-2AM calcium imaging to show that higher levels of PGE2 elevate intracellular calcium levels in the cell soma and growth cones of differentiated neuroectodermal (NE-4C) stem cells. PGE2 also increased the amplitude of calcium fluctuation in the neuronal growth cones and affected the neurite extension length. In summary, our results show that PGE2 may adversely impact intracellular calcium dynamics in differentiated neuronal cells and possibly affect early development of the nervous system.",

keywords = "Autism spectrum disorders, Calcium fluctuations, Differentiated neuroectodermal stem cells, Growth cone, Neurite extension, Neurons, Prostaglandin E2",

author = "Davidson, {Jennilee M.} and Wong, {Christine T.} and Ravneet Rai-Bhogal and Hongyan Li and Crawford, {Dorota A.}",

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doi = "10.1016/j.mcn.2016.03.010",

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journal = "Molecular and Cellular Neuroscience",

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T1 - Prostaglandin E2 elevates calcium in differentiated neuroectodermal stem cells

AU - Davidson, Jennilee M.

AU - Wong, Christine T.

AU - Rai-Bhogal, Ravneet

AU - Li, Hongyan

AU - Crawford, Dorota A.

PY - 2016/7/1

Y1 - 2016/7/1

N2 - Lipid mediator prostaglandin E2 (PGE2) is an endogenous signaling molecule that plays an important role during early development of the nervous system. Abnormalities in the PGE2 signaling pathway have been associated with neurodevelopmental disorders such as autism spectrum disorders. In this study we use ratiometric fura-2AM calcium imaging to show that higher levels of PGE2 elevate intracellular calcium levels in the cell soma and growth cones of differentiated neuroectodermal (NE-4C) stem cells. PGE2 also increased the amplitude of calcium fluctuation in the neuronal growth cones and affected the neurite extension length. In summary, our results show that PGE2 may adversely impact intracellular calcium dynamics in differentiated neuronal cells and possibly affect early development of the nervous system.

AB - Lipid mediator prostaglandin E2 (PGE2) is an endogenous signaling molecule that plays an important role during early development of the nervous system. Abnormalities in the PGE2 signaling pathway have been associated with neurodevelopmental disorders such as autism spectrum disorders. In this study we use ratiometric fura-2AM calcium imaging to show that higher levels of PGE2 elevate intracellular calcium levels in the cell soma and growth cones of differentiated neuroectodermal (NE-4C) stem cells. PGE2 also increased the amplitude of calcium fluctuation in the neuronal growth cones and affected the neurite extension length. In summary, our results show that PGE2 may adversely impact intracellular calcium dynamics in differentiated neuronal cells and possibly affect early development of the nervous system.

KW - Autism spectrum disorders

KW - Calcium fluctuations

KW - Differentiated neuroectodermal stem cells

KW - Growth cone

KW - Neurite extension

KW - Neurons

KW - Prostaglandin E2

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ER -

Prostaglandin E2 elevates calcium in differentiated neuroectodermal stem cells

Abstract

Keywords

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