Prostaglandin E2 elevates calcium in differentiated neuroectodermal stem cells

Jennilee M. Davidson, Christine T. Wong, Ravneet Rai-Bhogal, Hongyan Li, Dorota A. Crawford*

*Corresponding author for this work

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Lipid mediator prostaglandin E2 (PGE2) is an endogenous signaling molecule that plays an important role during early development of the nervous system. Abnormalities in the PGE2 signaling pathway have been associated with neurodevelopmental disorders such as autism spectrum disorders. In this study we use ratiometric fura-2AM calcium imaging to show that higher levels of PGE2 elevate intracellular calcium levels in the cell soma and growth cones of differentiated neuroectodermal (NE-4C) stem cells. PGE2 also increased the amplitude of calcium fluctuation in the neuronal growth cones and affected the neurite extension length. In summary, our results show that PGE2 may adversely impact intracellular calcium dynamics in differentiated neuronal cells and possibly affect early development of the nervous system.

Original languageEnglish
Pages (from-to)71-77
Number of pages7
JournalMolecular and Cellular Neuroscience
Volume74
DOIs
Publication statusPublished - 1 Jul 2016
Externally publishedYes

Keywords

  • Autism spectrum disorders
  • Calcium fluctuations
  • Differentiated neuroectodermal stem cells
  • Growth cone
  • Neurite extension
  • Neurons
  • Prostaglandin E2

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