Protective effects of myxobacterial extracts on hydrogen peroxide-induced toxicity on human primary astrocytes

Mona Dehhaghi, Vanessa Tan, Benjamin Heng, Fatemeh Mohammadipanah, Gilles J. Guillemin

Research output: Contribution to journalArticleResearchpeer-review

Abstract

Astrocytes, the main non-neuronal cells in the brain, have significant roles in the maintenance and survival of neurons. Oxidative stress has been implicated in various neurodegenerative disorders such as Alzheimer's disease (AD), amyotrophic lateral sclerosis (ALS), and Parkinson's disease (PD). Myxobacteria produce a wide range of bioactive metabolites with notable structures and modes of action, which introduce them as potent natural product producers. In the present study, we evaluated the effects of myxobacterial extracts on hydrogen peroxide (H2O2)-mediated toxicity on primary human astrocytes. We showed that myxobacterial extracts could decrease the formation of reactive oxygen species (ROS), nitric oxide (NO) production, and cell death assessed by the release of lactate dehydrogenase (LDH). Myxobacterial extracts were also able to reduce the nitric oxide synthase (NOS) activity. The extracts reduced the oxidative effect of H2O2 on over-activation of poly (ADP-ribose) polymerase (PARP1), therefore preventing the cell death by restoring the NAD+ levels. In addition, myxobacterial extracts ameliorated the oxidative stress by increasing the glutathione level in cells. The overall results showed myxobacterial extracts, especially from the strains Archangium sp. UTMC 4070 and Cystobacter sp. UTMC 4073, were able to protect human primary astrocytes from oxidative stress.

LanguageEnglish
Pages1-11
Number of pages11
JournalNeuroscience
Volume399
DOIs
Publication statusPublished - 10 Feb 2019

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Astrocytes
Hydrogen Peroxide
Oxidative Stress
Cell Death
Myxococcales
Poly(ADP-ribose) Polymerases
Amyotrophic Lateral Sclerosis
Biological Products
L-Lactate Dehydrogenase
Nitric Oxide Synthase
Neurodegenerative Diseases
NAD
Glutathione
Parkinson Disease
Reactive Oxygen Species
Alzheimer Disease
Nitric Oxide
Maintenance
Neurons
Brain

Keywords

  • glioprotection
  • myxobacteria
  • natural product
  • neurodegenerative diseases
  • oxidative stress

Cite this

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abstract = "Astrocytes, the main non-neuronal cells in the brain, have significant roles in the maintenance and survival of neurons. Oxidative stress has been implicated in various neurodegenerative disorders such as Alzheimer's disease (AD), amyotrophic lateral sclerosis (ALS), and Parkinson's disease (PD). Myxobacteria produce a wide range of bioactive metabolites with notable structures and modes of action, which introduce them as potent natural product producers. In the present study, we evaluated the effects of myxobacterial extracts on hydrogen peroxide (H2O2)-mediated toxicity on primary human astrocytes. We showed that myxobacterial extracts could decrease the formation of reactive oxygen species (ROS), nitric oxide (NO) production, and cell death assessed by the release of lactate dehydrogenase (LDH). Myxobacterial extracts were also able to reduce the nitric oxide synthase (NOS) activity. The extracts reduced the oxidative effect of H2O2 on over-activation of poly (ADP-ribose) polymerase (PARP1), therefore preventing the cell death by restoring the NAD+ levels. In addition, myxobacterial extracts ameliorated the oxidative stress by increasing the glutathione level in cells. The overall results showed myxobacterial extracts, especially from the strains Archangium sp. UTMC 4070 and Cystobacter sp. UTMC 4073, were able to protect human primary astrocytes from oxidative stress.",
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Protective effects of myxobacterial extracts on hydrogen peroxide-induced toxicity on human primary astrocytes. / Dehhaghi, Mona; Tan, Vanessa; Heng, Benjamin; Mohammadipanah, Fatemeh; Guillemin, Gilles J.

In: Neuroscience, Vol. 399, 10.02.2019, p. 1-11.

Research output: Contribution to journalArticleResearchpeer-review

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