Protein Glycosylation

J. J. Kattla*, W. B. Struwe, M. Doherty, B. Adamczyk, R. Saldova, P. M. Rudd, M. P. Campbell

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingChapter

2 Citations (Scopus)

Abstract

Protein glycosylation is a complex posttranslational modification that manipulates the biological activity and function of therapeutic glycoproteins. To date, a plethora of functions related to glycan composition have been described in the literature: from solubility, stability, cellular localization, molecular trafficking, self-recognition, clearance, transport, immunogenicity, and circulating half-life. Most recently, glycan composition has been the target of modification to enhance the safety and efficacy of glycoprotein therapeutics. These links between the complex nature of the glycans and their associated functions have raised serious concerns within the drug regulatory authorities across the world. Glycans are the most complex and heterogeneous class of molecules due to their non-template-driven biosynthetic process, consequently making glycan characterization difficult. There is no universal method available that can characterize the complete intact glycoprotein structure; it is essential to apply several orthogonal methods to measure individual parameters such as glycosylation site analysis, oligosaccharide sequence, and monosaccharide content of a therapeutic glycoprotein. In this article, we discuss various state-of-the-art glycoanalytical approaches and strategies for evaluating total glycoprotein structure and function.

Original languageEnglish
Title of host publicationIndustrial Biotechnology and Commodity Products
PublisherElsevier
Pages467-486
Number of pages20
Volume3
Edition2nd
ISBN (Electronic)9780080885049
ISBN (Print)9780444533524
DOIs
Publication statusPublished - 9 Sep 2011

Keywords

  • Biopharmaceutical glycosylation
  • Glycan analysis
  • Glycoanalytics
  • Glycomics
  • Glycoprofiling

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    Kattla, J. J., Struwe, W. B., Doherty, M., Adamczyk, B., Saldova, R., Rudd, P. M., & Campbell, M. P. (2011). Protein Glycosylation. In Industrial Biotechnology and Commodity Products (2nd ed., Vol. 3, pp. 467-486). Elsevier. https://doi.org/10.1016/B978-0-08-088504-9.00230-0