Protein microarray applications

Autoantibody detection and posttranslational modification

Apurva Atak, Shuvolina Mukherjee, Rekha Jain, Shabarni Gupta, Vedita Anand Singh, Nikita Gahoi, K. P. Manubhai, Sanjeeva Srivastava*

*Corresponding author for this work

Research output: Contribution to journalReview article

16 Citations (Scopus)

Abstract

The discovery of DNA microarrays was a major milestone in genomics; however, it could not adequately predict the structure or dynamics of underlying protein entities, which are the ultimate effector molecules in a cell. Protein microarrays allow simultaneous study of thousands of proteins/peptides, and various advancements in array technologies have made this platform suitable for several diagnostic and functional studies. Antibody arrays enable researchers to quantify the abundance of target proteins in biological fluids and assess PTMs by using the antibodies. Protein microarrays have been used to assess protein–protein interactions, protein–ligand interactions, and autoantibody profiling in various disease conditions. Here, we summarize different microarray platforms with focus on its biological and clinical applications in autoantibody profiling and PTM studies. We also enumerate the potential of tissue microarrays to validate findings from protein arrays as well as other approaches, highlighting their significance in proteomics.

Original languageEnglish
Pages (from-to)2557-2569
Number of pages13
JournalProteomics
Volume16
Issue number19
DOIs
Publication statusPublished - 1 Oct 2016
Externally publishedYes

Keywords

  • autoantibody profiling
  • protein arrays
  • PTM studies
  • tissue microarrays

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