Protein phosphorylation: technologies for the identification of phosphoamino acids

Jun X. Yan, Nicolle H. Packer, Andrew A. Gooley, Keith L. Williams*

*Corresponding author for this work

Research output: Contribution to journalReview articlepeer-review

146 Citations (Scopus)


Protein phosphorylation plays a central role in many biological and biomedical phenomena. In this, while a brief overview of the occurrence and function of protein phosphorylation is given, the primary focus is on studies related to the detection and analysis of phosphorylation both in vivo and in vitro. We focus on phosphorylation of serine, threonine and tyrosine, the most commonly phosphorylated amino acids in eukaryotes. Technologies such as radiolabelling, antibody recognition, chromatographic methods (HPLC, TLC), electrophoresis, Edman sequencing and mass spectrometry are reviewed. We consider the speed, simplicity and sensitivity of tools for detection and identification of protein phosphorylation, as well as quantitation and site characterisation. The limitations of currently available methods are summarised.

Original languageEnglish
Pages (from-to)23-41
Number of pages19
JournalJournal of Chromatography A
Issue number1-2
Publication statusPublished - 29 May 1998


  • amino acids
  • phosphoamino acids
  • reviews
  • serine
  • threonine
  • tyrosine

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