Proteins of rat serum: II. Influence of some biological parameters of the two-dimensional electrophoresis pattern

I. Miller; P. Haynes; M. Gemeiner; R. Aebersold; C. Manzoni; M. R. Lovti; M. Vignati; I. Eberini; E. Gianazza

doi:10.1002/elps.1150190846

Proteins of rat serum: II. Influence of some biological parameters of the two-dimensional electrophoresis pattern

I. Miller, P. Haynes, M. Gemeiner, R. Aebersold, C. Manzoni, M. R. Lovti, M. Vignati, I. Eberini, E. Gianazza^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

38 Citations (Scopus)

Abstract

This report complements the database already detailed for serum proteins of healthy adult male rats (P. Haynes et al., Electrophoresis 1998, 19, 1484-1492). The influence on the two-dimensional electrophoresis (2-DE) pattern of certain physiological conditions (sex, age) was studied as well as of changes in thyroid metabolism. We have extended the information about the major components of rat serum by identifying the proteins typical for the response to acute inflammation. Analyzing 27 spots, six proteins not found in control sera could be recognized; migration at overlapping or close positions with five already characterized proteins was observed for some. A compilation of all our rat data can be accessed through: http ://weber.u.washington.edu/ ~ruedilab/aebersold.htm1.

Original language	English
Pages (from-to)	1493-1500
Number of pages	8
Journal	Electrophoresis
Volume	19
Issue number	8-9
DOIs	https://doi.org/10.1002/elps.1150190846
Publication status	Published - 1998
Externally published	Yes

Keywords

Affinity chromatography
Inflammation
Rat serum
Thyroid
Two-dimensional polyacrylamide gel electrophoresis

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10.1002/elps.1150190846

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abstract = "This report complements the database already detailed for serum proteins of healthy adult male rats (P. Haynes et al., Electrophoresis 1998, 19, 1484-1492). The influence on the two-dimensional electrophoresis (2-DE) pattern of certain physiological conditions (sex, age) was studied as well as of changes in thyroid metabolism. We have extended the information about the major components of rat serum by identifying the proteins typical for the response to acute inflammation. Analyzing 27 spots, six proteins not found in control sera could be recognized; migration at overlapping or close positions with five already characterized proteins was observed for some. A compilation of all our rat data can be accessed through: http ://weber.u.washington.edu/ ~ruedilab/aebersold.htm1.",

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T2 - II. Influence of some biological parameters of the two-dimensional electrophoresis pattern

AU - Miller, I.

AU - Haynes, P.

AU - Gemeiner, M.

AU - Aebersold, R.

AU - Manzoni, C.

AU - Lovti, M. R.

AU - Vignati, M.

AU - Eberini, I.

AU - Gianazza, E.

PY - 1998

Y1 - 1998

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KW - Affinity chromatography

KW - Inflammation

KW - Rat serum

KW - Thyroid

KW - Two-dimensional polyacrylamide gel electrophoresis

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Proteins of rat serum: II. Influence of some biological parameters of the two-dimensional electrophoresis pattern

Abstract

Keywords

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