Proteins of rat serum

II. Influence of some biological parameters of the two-dimensional electrophoresis pattern

I. Miller, P. Haynes, M. Gemeiner, R. Aebersold, C. Manzoni, M. R. Lovti, M. Vignati, I. Eberini, E. Gianazza*

*Corresponding author for this work

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Abstract

This report complements the database already detailed for serum proteins of healthy adult male rats (P. Haynes et al., Electrophoresis 1998, 19, 1484-1492). The influence on the two-dimensional electrophoresis (2-DE) pattern of certain physiological conditions (sex, age) was studied as well as of changes in thyroid metabolism. We have extended the information about the major components of rat serum by identifying the proteins typical for the response to acute inflammation. Analyzing 27 spots, six proteins not found in control sera could be recognized; migration at overlapping or close positions with five already characterized proteins was observed for some. A compilation of all our rat data can be accessed through: http ://weber.u.washington.edu/ ~ruedilab/aebersold.htm1.

Original languageEnglish
Pages (from-to)1493-1500
Number of pages8
JournalElectrophoresis
Volume19
Issue number8-9
DOIs
Publication statusPublished - 1998
Externally publishedYes

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Keywords

  • Affinity chromatography
  • Inflammation
  • Rat serum
  • Thyroid
  • Two-dimensional polyacrylamide gel electrophoresis

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