Proteins of rat serum: III. Gender-related differences in protein concentration under baseline conditions and upon experimental inflammation as evaluated by two-dimensional electrophoresis

Ingrid Miller, Paul Haynes, Ivano Eberlni, Manfred Gemeiner, Ruedi Aebersold, Elisabetta Gianazza*

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

Abstract

We have previously described the major components of rat serum (Electrophoresis 1998, 19, 1484-1492 and 1493-1500). In this report we examine sex-related differences in protein concentrations, both in control animals and upon experimentally induced inflammation. Under baseline conditions approximately one third of the spots resolved in serum by two-dimensional electrophoresis (2-DE) are expressed at levels ≥ 25% higher in female rats than in male rats and a further 10% at levels ≥ 25% lower. Inflammation increases the expression of the positive acute-phase reactants: hemopexin, ceruloplasmin, α1-antitrypsin (all approximately 2-fold), C-reactive protein (3- to 5-fold), serine protease inhibitor-3 (4- to 5-fold), thiostatin (> 5-fold in females, > 20-fold in males), clusterin, orosomucoid, haptoglobin chains and α2-macroglobulin. The baseline level of the last four markers is below the detection limit, hence no percent increase can be computed. Conversely, negative acute-phase reactants are reduced on inflammation: α1-inhibitor III, α2-HS-glycoprotein, kallikrein-binding protein and transthyretin (all reduced to between 1/2 to 1/3 of the baseline levels), retinol-binding protein (to about 1/2 to 1/4) and albumin (to 2/3). Except for thiostatin, the changes in acute-phase protein levels are similar in male and female rats.

Original languageEnglish
Title of host publicationFrom genome to proteome
Subtitle of host publication advances in the practice & application of proteomics
EditorsMichael Dunn
Place of PublicationWeinheim, Germany; New York
PublisherWiley-Blackwell, Wiley
Pages256-265
Number of pages10
ISBN (Electronic)9783527613489
ISBN (Print)9783527301546, 9783527613496, 3527301542
DOIs
Publication statusPublished - 26 Dec 2007
Externally publishedYes

Keywords

  • Acute-phase reactants
  • Inflammation
  • Rat serum
  • Sex
  • Two-dimensional Polyacrylamide gel electrophoresis

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