TY - JOUR
T1 - Proteome profiling of enriched membrane-associated proteins unraveled a novel sophorose and cello-oligosaccharide transporter in Trichoderma reesei
AU - Nogueira, Karoline Maria Vieira
AU - Mendes, Vanessa
AU - Kamath, Karthik Shantharam
AU - Cheruku, Anusha
AU - Oshiquiri, Letícia Harumi
AU - de Paula, Renato Graciano
AU - Carraro, Claudia
AU - Pedersoli, Wellington Ramos
AU - Pereira, Lucas Matheus Soares
AU - Vieira, Luiz Carlos
AU - Steindorff, Andrei Stecca
AU - Amirkhani, Ardeshir
AU - McKay, Matthew J.
AU - Nevalainen, Helena
AU - Molloy, Mark P.
AU - Silva, Roberto N.
N1 - Copyright the Author(s) 2024. Version archived for private and non-commercial use with the permission of the author/s and according to publisher conditions. For further rights please contact the publisher.
PY - 2024/1/16
Y1 - 2024/1/16
N2 - Background: Trichoderma reesei is an organism extensively used in the bioethanol industry, owing to its capability to produce enzymes capable of breaking down holocellulose into simple sugars. The uptake of carbohydrates generated from cellulose breakdown is crucial to induce the signaling cascade that triggers cellulase production. However, the sugar transporters involved in this process in T. reesei remain poorly identified and characterized. Results: To address this gap, this study used temporal membrane proteomics analysis to identify five known and nine putative sugar transporters that may be involved in cellulose degradation by T. reesei. Docking analysis pointed out potential ligands for the putative sugar transporter Tr44175. Further functional validation of this transporter was carried out in Saccharomyces cerevisiae. The results showed that Tr44175 transports a variety of sugar molecules, including cellobiose, cellotriose, cellotetraose, and sophorose. Conclusion: This study has unveiled a transporter Tr44175 capable of transporting cellobiose, cellotriose, cellotetraose, and sophorose. Our study represents the first inventory of T. reesei sugar transportome once exposed to cellulose, offering promising potential targets for strain engineering in the context of bioethanol production.
AB - Background: Trichoderma reesei is an organism extensively used in the bioethanol industry, owing to its capability to produce enzymes capable of breaking down holocellulose into simple sugars. The uptake of carbohydrates generated from cellulose breakdown is crucial to induce the signaling cascade that triggers cellulase production. However, the sugar transporters involved in this process in T. reesei remain poorly identified and characterized. Results: To address this gap, this study used temporal membrane proteomics analysis to identify five known and nine putative sugar transporters that may be involved in cellulose degradation by T. reesei. Docking analysis pointed out potential ligands for the putative sugar transporter Tr44175. Further functional validation of this transporter was carried out in Saccharomyces cerevisiae. The results showed that Tr44175 transports a variety of sugar molecules, including cellobiose, cellotriose, cellotetraose, and sophorose. Conclusion: This study has unveiled a transporter Tr44175 capable of transporting cellobiose, cellotriose, cellotetraose, and sophorose. Our study represents the first inventory of T. reesei sugar transportome once exposed to cellulose, offering promising potential targets for strain engineering in the context of bioethanol production.
KW - Cellulose
KW - Membrane-associated proteome
KW - Sugar transporters
KW - Trichoderma reesei
UR - http://www.scopus.com/inward/record.url?scp=85182480175&partnerID=8YFLogxK
U2 - 10.1186/s12934-023-02279-9
DO - 10.1186/s12934-023-02279-9
M3 - Article
C2 - 38229067
AN - SCOPUS:85182480175
SN - 1475-2859
VL - 23
SP - 1
EP - 16
JO - Microbial Cell Factories
JF - Microbial Cell Factories
IS - 1
M1 - 22
ER -