Proteome profiling of enriched membrane-associated proteins unraveled a novel sophorose and cello-oligosaccharide transporter in Trichoderma reesei

Karoline Maria Vieira Nogueira; Vanessa Mendes; Karthik Shantharam Kamath; Anusha Cheruku; Letícia Harumi Oshiquiri; Renato Graciano de Paula; Claudia Carraro; Wellington Ramos Pedersoli; Lucas Matheus Soares Pereira; Luiz Carlos Vieira; Andrei Stecca Steindorff; Ardeshir Amirkhani; Matthew J. McKay; Helena Nevalainen; Mark P. Molloy; Roberto N. Silva

doi:10.1186/s12934-023-02279-9

Proteome profiling of enriched membrane-associated proteins unraveled a novel sophorose and cello-oligosaccharide transporter in Trichoderma reesei

Karoline Maria Vieira Nogueira, Vanessa Mendes, Karthik Shantharam Kamath, Anusha Cheruku, Letícia Harumi Oshiquiri, Renato Graciano de Paula, Claudia Carraro, Wellington Ramos Pedersoli, Lucas Matheus Soares Pereira, Luiz Carlos Vieira, Andrei Stecca Steindorff, Ardeshir Amirkhani, Matthew J. McKay, Helena Nevalainen, Mark P. Molloy, Roberto N. Silva^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

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Abstract

Background: Trichoderma reesei is an organism extensively used in the bioethanol industry, owing to its capability to produce enzymes capable of breaking down holocellulose into simple sugars. The uptake of carbohydrates generated from cellulose breakdown is crucial to induce the signaling cascade that triggers cellulase production. However, the sugar transporters involved in this process in T. reesei remain poorly identified and characterized.

Results: To address this gap, this study used temporal membrane proteomics analysis to identify five known and nine putative sugar transporters that may be involved in cellulose degradation by T. reesei. Docking analysis pointed out potential ligands for the putative sugar transporter Tr44175. Further functional validation of this transporter was carried out in Saccharomyces cerevisiae. The results showed that Tr44175 transports a variety of sugar molecules, including cellobiose, cellotriose, cellotetraose, and sophorose.

Conclusion: This study has unveiled a transporter Tr44175 capable of transporting cellobiose, cellotriose, cellotetraose, and sophorose. Our study represents the first inventory of T. reesei sugar transportome once exposed to cellulose, offering promising potential targets for strain engineering in the context of bioethanol production.

Original language	English
Article number	22
Pages (from-to)	1-16
Number of pages	16
Journal	Microbial Cell Factories
Volume	23
Issue number	1
DOIs	https://doi.org/10.1186/s12934-023-02279-9
Publication status	Published - 16 Jan 2024

Bibliographical note

Copyright the Author(s) 2024. Version archived for private and non-commercial use with the permission of the author/s and according to publisher conditions. For further rights please contact the publisher.

Keywords

Cellulose
Membrane-associated proteome
Sugar transporters
Trichoderma reesei

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10.1186/s12934-023-02279-9

Publisher version (open access)Final published version, 3.02 MBLicence: CC BY

Cite this

Nogueira, K. M. V., Mendes, V., Kamath, K. S., Cheruku, A., Oshiquiri, L. H., de Paula, R. G., Carraro, C., Pedersoli, W. R., Pereira, L. M. S., Vieira, L. C., Steindorff, A. S., Amirkhani, A., McKay, M. J., Nevalainen, H., Molloy, M. P., & Silva, R. N. (2024). Proteome profiling of enriched membrane-associated proteins unraveled a novel sophorose and cello-oligosaccharide transporter in Trichoderma reesei. Microbial Cell Factories, 23(1), 1-16. Article 22. https://doi.org/10.1186/s12934-023-02279-9

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title = "Proteome profiling of enriched membrane-associated proteins unraveled a novel sophorose and cello-oligosaccharide transporter in Trichoderma reesei",

abstract = "Background: Trichoderma reesei is an organism extensively used in the bioethanol industry, owing to its capability to produce enzymes capable of breaking down holocellulose into simple sugars. The uptake of carbohydrates generated from cellulose breakdown is crucial to induce the signaling cascade that triggers cellulase production. However, the sugar transporters involved in this process in T. reesei remain poorly identified and characterized. Results: To address this gap, this study used temporal membrane proteomics analysis to identify five known and nine putative sugar transporters that may be involved in cellulose degradation by T. reesei. Docking analysis pointed out potential ligands for the putative sugar transporter Tr44175. Further functional validation of this transporter was carried out in Saccharomyces cerevisiae. The results showed that Tr44175 transports a variety of sugar molecules, including cellobiose, cellotriose, cellotetraose, and sophorose. Conclusion: This study has unveiled a transporter Tr44175 capable of transporting cellobiose, cellotriose, cellotetraose, and sophorose. Our study represents the first inventory of T. reesei sugar transportome once exposed to cellulose, offering promising potential targets for strain engineering in the context of bioethanol production.",

keywords = "Cellulose, Membrane-associated proteome, Sugar transporters, Trichoderma reesei",

author = "Nogueira, \{Karoline Maria Vieira\} and Vanessa Mendes and Kamath, \{Karthik Shantharam\} and Anusha Cheruku and Oshiquiri, \{Let{\'i}cia Harumi\} and \{de Paula\}, \{Renato Graciano\} and Claudia Carraro and Pedersoli, \{Wellington Ramos\} and Pereira, \{Lucas Matheus Soares\} and Vieira, \{Luiz Carlos\} and Steindorff, \{Andrei Stecca\} and Ardeshir Amirkhani and McKay, \{Matthew J.\} and Helena Nevalainen and Molloy, \{Mark P.\} and Silva, \{Roberto N.\}",

note = "Copyright the Author(s) 2024. Version archived for private and non-commercial use with the permission of the author/s and according to publisher conditions. For further rights please contact the publisher. ",

year = "2024",

month = jan,

day = "16",

doi = "10.1186/s12934-023-02279-9",

language = "English",

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Nogueira, KMV, Mendes, V, Kamath, KS, Cheruku, A, Oshiquiri, LH, de Paula, RG, Carraro, C, Pedersoli, WR, Pereira, LMS, Vieira, LC, Steindorff, AS, Amirkhani, A, McKay, MJ, Nevalainen, H , Molloy, MP & Silva, RN 2024, 'Proteome profiling of enriched membrane-associated proteins unraveled a novel sophorose and cello-oligosaccharide transporter in Trichoderma reesei', Microbial Cell Factories, vol. 23, no. 1, 22, pp. 1-16. https://doi.org/10.1186/s12934-023-02279-9

Proteome profiling of enriched membrane-associated proteins unraveled a novel sophorose and cello-oligosaccharide transporter in Trichoderma reesei. / Nogueira, Karoline Maria Vieira; Mendes, Vanessa; Kamath, Karthik Shantharam et al.
In: Microbial Cell Factories, Vol. 23, No. 1, 22, 16.01.2024, p. 1-16.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Proteome profiling of enriched membrane-associated proteins unraveled a novel sophorose and cello-oligosaccharide transporter in Trichoderma reesei

AU - Nogueira, Karoline Maria Vieira

AU - Mendes, Vanessa

AU - Kamath, Karthik Shantharam

AU - Cheruku, Anusha

AU - Oshiquiri, Letícia Harumi

AU - de Paula, Renato Graciano

AU - Carraro, Claudia

AU - Pedersoli, Wellington Ramos

AU - Pereira, Lucas Matheus Soares

AU - Vieira, Luiz Carlos

AU - Steindorff, Andrei Stecca

AU - Amirkhani, Ardeshir

AU - McKay, Matthew J.

AU - Nevalainen, Helena

AU - Molloy, Mark P.

AU - Silva, Roberto N.

N1 - Copyright the Author(s) 2024. Version archived for private and non-commercial use with the permission of the author/s and according to publisher conditions. For further rights please contact the publisher.

PY - 2024/1/16

Y1 - 2024/1/16

N2 - Background: Trichoderma reesei is an organism extensively used in the bioethanol industry, owing to its capability to produce enzymes capable of breaking down holocellulose into simple sugars. The uptake of carbohydrates generated from cellulose breakdown is crucial to induce the signaling cascade that triggers cellulase production. However, the sugar transporters involved in this process in T. reesei remain poorly identified and characterized. Results: To address this gap, this study used temporal membrane proteomics analysis to identify five known and nine putative sugar transporters that may be involved in cellulose degradation by T. reesei. Docking analysis pointed out potential ligands for the putative sugar transporter Tr44175. Further functional validation of this transporter was carried out in Saccharomyces cerevisiae. The results showed that Tr44175 transports a variety of sugar molecules, including cellobiose, cellotriose, cellotetraose, and sophorose. Conclusion: This study has unveiled a transporter Tr44175 capable of transporting cellobiose, cellotriose, cellotetraose, and sophorose. Our study represents the first inventory of T. reesei sugar transportome once exposed to cellulose, offering promising potential targets for strain engineering in the context of bioethanol production.

AB - Background: Trichoderma reesei is an organism extensively used in the bioethanol industry, owing to its capability to produce enzymes capable of breaking down holocellulose into simple sugars. The uptake of carbohydrates generated from cellulose breakdown is crucial to induce the signaling cascade that triggers cellulase production. However, the sugar transporters involved in this process in T. reesei remain poorly identified and characterized. Results: To address this gap, this study used temporal membrane proteomics analysis to identify five known and nine putative sugar transporters that may be involved in cellulose degradation by T. reesei. Docking analysis pointed out potential ligands for the putative sugar transporter Tr44175. Further functional validation of this transporter was carried out in Saccharomyces cerevisiae. The results showed that Tr44175 transports a variety of sugar molecules, including cellobiose, cellotriose, cellotetraose, and sophorose. Conclusion: This study has unveiled a transporter Tr44175 capable of transporting cellobiose, cellotriose, cellotetraose, and sophorose. Our study represents the first inventory of T. reesei sugar transportome once exposed to cellulose, offering promising potential targets for strain engineering in the context of bioethanol production.

KW - Cellulose

KW - Membrane-associated proteome

KW - Sugar transporters

KW - Trichoderma reesei

UR - https://www.scopus.com/pages/publications/85182480175

U2 - 10.1186/s12934-023-02279-9

DO - 10.1186/s12934-023-02279-9

M3 - Article

C2 - 38229067

AN - SCOPUS:85182480175

SN - 1475-2859

VL - 23

SP - 1

EP - 16

JO - Microbial Cell Factories

JF - Microbial Cell Factories

IS - 1

M1 - 22

ER -

Proteome profiling of enriched membrane-associated proteins unraveled a novel sophorose and cello-oligosaccharide transporter in Trichoderma reesei

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