Abstract
Background: The opportunistic pathogen, Pseudomonas aeruginosa is well known for its environmental and metabolic versatility, yet many of the functions of its gene-products remain to be fully elucidated. This study's objective was to illuminate the potential functions of under-described gene-products during the medically relevant copper-stress condition.
Results: We used data-independent acquisition mass spectrometry to quantitate protein expression changes associated with copper stress in P. aeruginosa PAO1. Approximately 2000 non-redundant proteins were quantified, with 78 proteins altering in abundance by +/- 1.5-fold or more when cultured to mid-log growth in the presence of 50 μM copper sulfate. One-third of those differentially expressed proteins have no prior established functional roles.
Conclusions: This study provides evidence for the functional involvement of some specific proteins in enabling P. aeruginosa to survive under sub-lethal concentrations of copper. This further paves the way for targeted investigations into the specific mechanisms of their activity.
| Language | English |
|---|---|
| Article number | 69 |
| Number of pages | 13 |
| Journal | BMC Microbiology |
| Volume | 19 |
| DOIs | |
| Publication status | Published - 1 Apr 2019 |
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Bibliographical note
Copyright the Author(s) 2019. Version archived for private and non-commercial use with the permission of the author/s and according to publisher conditions. For further rights please contact the publisher.Keywords
- Mass spectrometry
- Copper stress
- Proteome
- Pseudomonas aeruginosa
Cite this
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Proteome profiling of Pseudomonas aeruginosa PAO1 identifies novel responders to copper stress. / Wright, Bradley W.; Kamath, Karthik S.; Krisp, Christoph; Molloy, Mark P.
In: BMC Microbiology, Vol. 19, 69, 01.04.2019.Research output: Contribution to journal › Article › Research › peer-review
TY - JOUR
T1 - Proteome profiling of Pseudomonas aeruginosa PAO1 identifies novel responders to copper stress
AU - Wright, Bradley W.
AU - Kamath, Karthik S.
AU - Krisp, Christoph
AU - Molloy, Mark P.
N1 - Copyright the Author(s) 2019. Version archived for private and non-commercial use with the permission of the author/s and according to publisher conditions. For further rights please contact the publisher.
PY - 2019/4/1
Y1 - 2019/4/1
N2 - Background: The opportunistic pathogen, Pseudomonas aeruginosa is well known for its environmental and metabolic versatility, yet many of the functions of its gene-products remain to be fully elucidated. This study's objective was to illuminate the potential functions of under-described gene-products during the medically relevant copper-stress condition. Results: We used data-independent acquisition mass spectrometry to quantitate protein expression changes associated with copper stress in P. aeruginosa PAO1. Approximately 2000 non-redundant proteins were quantified, with 78 proteins altering in abundance by +/- 1.5-fold or more when cultured to mid-log growth in the presence of 50 μM copper sulfate. One-third of those differentially expressed proteins have no prior established functional roles. Conclusions: This study provides evidence for the functional involvement of some specific proteins in enabling P. aeruginosa to survive under sub-lethal concentrations of copper. This further paves the way for targeted investigations into the specific mechanisms of their activity.
AB - Background: The opportunistic pathogen, Pseudomonas aeruginosa is well known for its environmental and metabolic versatility, yet many of the functions of its gene-products remain to be fully elucidated. This study's objective was to illuminate the potential functions of under-described gene-products during the medically relevant copper-stress condition. Results: We used data-independent acquisition mass spectrometry to quantitate protein expression changes associated with copper stress in P. aeruginosa PAO1. Approximately 2000 non-redundant proteins were quantified, with 78 proteins altering in abundance by +/- 1.5-fold or more when cultured to mid-log growth in the presence of 50 μM copper sulfate. One-third of those differentially expressed proteins have no prior established functional roles. Conclusions: This study provides evidence for the functional involvement of some specific proteins in enabling P. aeruginosa to survive under sub-lethal concentrations of copper. This further paves the way for targeted investigations into the specific mechanisms of their activity.
KW - Mass spectrometry
KW - Copper stress
KW - Proteome
KW - Pseudomonas aeruginosa
UR - http://www.scopus.com/inward/record.url?scp=85063750469&partnerID=8YFLogxK
U2 - 10.1186/s12866-019-1441-7
DO - 10.1186/s12866-019-1441-7
M3 - Article
VL - 19
JO - BMC Microbiology
T2 - BMC Microbiology
JF - BMC Microbiology
SN - 1471-2180
M1 - 69
ER -