Proteome profiling of Pseudomonas aeruginosa PAO1 identifies novel responders to copper stress

Research output: Contribution to journalArticleResearchpeer-review

Abstract

Background: The opportunistic pathogen, Pseudomonas aeruginosa is well known for its environmental and metabolic versatility, yet many of the functions of its gene-products remain to be fully elucidated. This study's objective was to illuminate the potential functions of under-described gene-products during the medically relevant copper-stress condition.

Results: We used data-independent acquisition mass spectrometry to quantitate protein expression changes associated with copper stress in P. aeruginosa PAO1. Approximately 2000 non-redundant proteins were quantified, with 78 proteins altering in abundance by +/- 1.5-fold or more when cultured to mid-log growth in the presence of 50 μM copper sulfate. One-third of those differentially expressed proteins have no prior established functional roles.

Conclusions: This study provides evidence for the functional involvement of some specific proteins in enabling P. aeruginosa to survive under sub-lethal concentrations of copper. This further paves the way for targeted investigations into the specific mechanisms of their activity.

LanguageEnglish
Article number69
Pages1-13
Number of pages13
JournalBMC Microbiology
Volume19
DOIs
Publication statusPublished - 1 Apr 2019

Fingerprint

Proteome
Pseudomonas aeruginosa
Copper
Proteins
Copper Sulfate
Genes
Mass Spectrometry
Growth

Bibliographical note

Copyright the Author(s) 2019. Version archived for private and non-commercial use with the permission of the author/s and according to publisher conditions. For further rights please contact the publisher.

Keywords

  • Mass spectrometry
  • Copper stress
  • Proteome
  • Pseudomonas aeruginosa

Cite this

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title = "Proteome profiling of Pseudomonas aeruginosa PAO1 identifies novel responders to copper stress",
abstract = "Background: The opportunistic pathogen, Pseudomonas aeruginosa is well known for its environmental and metabolic versatility, yet many of the functions of its gene-products remain to be fully elucidated. This study's objective was to illuminate the potential functions of under-described gene-products during the medically relevant copper-stress condition. Results: We used data-independent acquisition mass spectrometry to quantitate protein expression changes associated with copper stress in P. aeruginosa PAO1. Approximately 2000 non-redundant proteins were quantified, with 78 proteins altering in abundance by +/- 1.5-fold or more when cultured to mid-log growth in the presence of 50 μM copper sulfate. One-third of those differentially expressed proteins have no prior established functional roles. Conclusions: This study provides evidence for the functional involvement of some specific proteins in enabling P. aeruginosa to survive under sub-lethal concentrations of copper. This further paves the way for targeted investigations into the specific mechanisms of their activity.",
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Proteome profiling of Pseudomonas aeruginosa PAO1 identifies novel responders to copper stress. / Wright, Bradley W.; Kamath, Karthik S.; Krisp, Christoph; Molloy, Mark P.

In: BMC Microbiology, Vol. 19, 69, 01.04.2019, p. 1-13.

Research output: Contribution to journalArticleResearchpeer-review

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