Proteome profiling of Pseudomonas aeruginosa PAO1 identifies novel responders to copper stress

Bradley W. Wright; Karthik S. Kamath; Christoph Krisp; Mark P. Molloy

doi:10.1186/s12866-019-1441-7

Proteome profiling of Pseudomonas aeruginosa PAO1 identifies novel responders to copper stress

Bradley W. Wright, Karthik S. Kamath, Christoph Krisp, Mark P. Molloy^*

^*Corresponding author for this work

Australian Proteome Analysis Facility (APAF)

Research output: Contribution to journal › Article › peer-review

23 Citations (Scopus)

107 Downloads (Pure)

Abstract

Background: The opportunistic pathogen, Pseudomonas aeruginosa is well known for its environmental and metabolic versatility, yet many of the functions of its gene-products remain to be fully elucidated. This study's objective was to illuminate the potential functions of under-described gene-products during the medically relevant copper-stress condition.

Results: We used data-independent acquisition mass spectrometry to quantitate protein expression changes associated with copper stress in P. aeruginosa PAO1. Approximately 2000 non-redundant proteins were quantified, with 78 proteins altering in abundance by +/- 1.5-fold or more when cultured to mid-log growth in the presence of 50 μM copper sulfate. One-third of those differentially expressed proteins have no prior established functional roles.

Conclusions: This study provides evidence for the functional involvement of some specific proteins in enabling P. aeruginosa to survive under sub-lethal concentrations of copper. This further paves the way for targeted investigations into the specific mechanisms of their activity.

Original language	English
Article number	69
Pages (from-to)	1-13
Number of pages	13
Journal	BMC Microbiology
Volume	19
DOIs	https://doi.org/10.1186/s12866-019-1441-7
Publication status	Published - 1 Apr 2019

Bibliographical note

Copyright the Author(s) 2019. Version archived for private and non-commercial use with the permission of the author/s and according to publisher conditions. For further rights please contact the publisher.

Keywords

Mass spectrometry
Copper stress
Proteome
Pseudomonas aeruginosa

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10.1186/s12866-019-1441-7Licence: CC BY

Publisher version (open access)Final published version, 878 KBLicence: CC BY

Cite this

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title = "Proteome profiling of Pseudomonas aeruginosa PAO1 identifies novel responders to copper stress",

abstract = "Background: The opportunistic pathogen, Pseudomonas aeruginosa is well known for its environmental and metabolic versatility, yet many of the functions of its gene-products remain to be fully elucidated. This study's objective was to illuminate the potential functions of under-described gene-products during the medically relevant copper-stress condition. Results: We used data-independent acquisition mass spectrometry to quantitate protein expression changes associated with copper stress in P. aeruginosa PAO1. Approximately 2000 non-redundant proteins were quantified, with 78 proteins altering in abundance by +/- 1.5-fold or more when cultured to mid-log growth in the presence of 50 μM copper sulfate. One-third of those differentially expressed proteins have no prior established functional roles. Conclusions: This study provides evidence for the functional involvement of some specific proteins in enabling P. aeruginosa to survive under sub-lethal concentrations of copper. This further paves the way for targeted investigations into the specific mechanisms of their activity.",

keywords = "Mass spectrometry, Copper stress, Proteome, Pseudomonas aeruginosa",

author = "Wright, \{Bradley W.\} and Kamath, \{Karthik S.\} and Christoph Krisp and Molloy, \{Mark P.\}",

note = "Copyright the Author(s) 2019. Version archived for private and non-commercial use with the permission of the author/s and according to publisher conditions. For further rights please contact the publisher.",

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doi = "10.1186/s12866-019-1441-7",

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AU - Wright, Bradley W.

AU - Kamath, Karthik S.

AU - Krisp, Christoph

AU - Molloy, Mark P.

N1 - Copyright the Author(s) 2019. Version archived for private and non-commercial use with the permission of the author/s and according to publisher conditions. For further rights please contact the publisher.

PY - 2019/4/1

Y1 - 2019/4/1

N2 - Background: The opportunistic pathogen, Pseudomonas aeruginosa is well known for its environmental and metabolic versatility, yet many of the functions of its gene-products remain to be fully elucidated. This study's objective was to illuminate the potential functions of under-described gene-products during the medically relevant copper-stress condition. Results: We used data-independent acquisition mass spectrometry to quantitate protein expression changes associated with copper stress in P. aeruginosa PAO1. Approximately 2000 non-redundant proteins were quantified, with 78 proteins altering in abundance by +/- 1.5-fold or more when cultured to mid-log growth in the presence of 50 μM copper sulfate. One-third of those differentially expressed proteins have no prior established functional roles. Conclusions: This study provides evidence for the functional involvement of some specific proteins in enabling P. aeruginosa to survive under sub-lethal concentrations of copper. This further paves the way for targeted investigations into the specific mechanisms of their activity.

AB - Background: The opportunistic pathogen, Pseudomonas aeruginosa is well known for its environmental and metabolic versatility, yet many of the functions of its gene-products remain to be fully elucidated. This study's objective was to illuminate the potential functions of under-described gene-products during the medically relevant copper-stress condition. Results: We used data-independent acquisition mass spectrometry to quantitate protein expression changes associated with copper stress in P. aeruginosa PAO1. Approximately 2000 non-redundant proteins were quantified, with 78 proteins altering in abundance by +/- 1.5-fold or more when cultured to mid-log growth in the presence of 50 μM copper sulfate. One-third of those differentially expressed proteins have no prior established functional roles. Conclusions: This study provides evidence for the functional involvement of some specific proteins in enabling P. aeruginosa to survive under sub-lethal concentrations of copper. This further paves the way for targeted investigations into the specific mechanisms of their activity.

KW - Mass spectrometry

KW - Copper stress

KW - Proteome

KW - Pseudomonas aeruginosa

UR - https://www.scopus.com/pages/publications/85063750469

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DO - 10.1186/s12866-019-1441-7

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AN - SCOPUS:85063750469

SN - 1471-2180

VL - 19

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EP - 13

JO - BMC Microbiology

JF - BMC Microbiology

M1 - 69

ER -

Proteome profiling of Pseudomonas aeruginosa PAO1 identifies novel responders to copper stress

Abstract

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Keywords

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