Proteome profiling - Pitfalls and progress

Paul A. Haynes, John R. Yates

Research output: Contribution to journalArticle

99 Citations (Scopus)

Abstract

In this review we examine the current state of analytical methods in proteomics. The conventional methodology using two-dimensional clectrophoresis gels and mass spectrometry is discussed, with particular reference to the advantages and shortcomings thereof. Two recently published methods which offer an alternative approach are presented and discussed, with emphasis on how they can provide information not available via twodimensional gel clectrophoresis. These two methods arc the isotope-coded affinity tags approach of Gygi et al. and the two-dimensional liquid chromatograpliy-tandem mass spectrometry approach as presented by Link et al. We conclude that both of these new techniques represent significant advances in analytical methodology for proteomc analysis. Furthermore, we believe that in the future biological research will continue to be enhanced by the continuation of such developments in proteomic analytical technology.

Original languageEnglish
Pages (from-to)81-87
Number of pages7
JournalYeast
Volume17
Issue number2
Publication statusPublished - 2000
Externally publishedYes

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    Haynes, P. A., & Yates, J. R. (2000). Proteome profiling - Pitfalls and progress. Yeast, 17(2), 81-87.